Title

Combined phase-sensitive acoustic microscopy and confocal laser scanning microscopy

Authors

Authors

A. Kamanyi; W. Ngwa; T. Betz; R. Wannemacher;W. Grill

Comments

Authors: contact us about adding a copy of your work at STARS@ucf.edu

Abbreviated Journal Title

Ultrasonics

Keywords

phase-sensitive acoustic microscopy; confocal laser scanning microscopy; polymer blend; cells; ELASTIC PROPERTIES; THIN-FILMS; Acoustics; Radiology, Nuclear Medicine & Medical Imaging

Abstract

Combined phase- sensitive acoustic microscopy ( PSAM) at 1.2 GHz and confocal laser scanning microscopy ( CLSM) in reflection and fluorescence has been implemented and applied to polymer blend films and fluorescently labeled. broblasts and neuronal cells in order to explore the prospects and the various contrast mechanisms of this powerful technique. Topographic contrast is available for appropriate samples from CLSM in reflection and, with significantly higher precision, from the acoustic phase images. Material contrast can be gained from acoustic amplitude V( z) graphs. In the case of the biological cells investigated, the optical and acoustic images are very different and exhibit different features of the samples. (c) 2006 Elsevier B. V. All rights reserved.

Journal Title

Ultrasonics

Volume

44

Publication Date

1-1-2006

Document Type

Article; Proceedings Paper

Language

English

First Page

E1295

Last Page

E1300

WOS Identifier

WOS:000248802400241

ISSN

0041-624X

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