Combined phase-sensitive acoustic microscopy and confocal laser scanning microscopy

    Authors

    A. Kamanyi; W. Ngwa; T. Betz; R. Wannemacher;W. Grill

    Comments

    Authors: contact us about adding a copy of your work at STARS@ucf.edu

    Abbreviated Journal Title

    Ultrasonics

    Keywords

    phase-sensitive acoustic microscopy; confocal laser scanning microscopy; polymer blend; cells; ELASTIC PROPERTIES; THIN-FILMS; Acoustics; Radiology, Nuclear Medicine & Medical Imaging

    Abstract

    Combined phase- sensitive acoustic microscopy ( PSAM) at 1.2 GHz and confocal laser scanning microscopy ( CLSM) in reflection and fluorescence has been implemented and applied to polymer blend films and fluorescently labeled. broblasts and neuronal cells in order to explore the prospects and the various contrast mechanisms of this powerful technique. Topographic contrast is available for appropriate samples from CLSM in reflection and, with significantly higher precision, from the acoustic phase images. Material contrast can be gained from acoustic amplitude V( z) graphs. In the case of the biological cells investigated, the optical and acoustic images are very different and exhibit different features of the samples. (c) 2006 Elsevier B. V. All rights reserved.

    Journal Title

    Ultrasonics

    Volume

    44

    Publication Date

    1-1-2006

    Document Type

    Article; Proceedings Paper

    Language

    English

    First Page

    E1295

    Last Page

    E1300

    WOS Identifier

    WOS:000248802400241

    ISSN

    0041-624X

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