Chloroplast-derived vaccine antigens and other therapeutic proteins

    Authors

    H. Daniell; S. Chebolu; S. Kumar; M. Singleton;R. Falconer

    Comments

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    Abbreviated Journal Title

    Vaccine

    Keywords

    chloroplast genetic engineering; plant made biopharmaceuticals; oral; delivery; TRANSGENIC CHLOROPLASTS; EXPRESSION; TOBACCO; GENE; TOLERANCE; PEPTIDE; Immunology; Medicine, Research & Experimental

    Abstract

    The chloroplast genetic engineering offers a number of unique advantages including high level of transgene expression, multi-gene expression in single transformation event and transgene containment due to maternal inheritance. Hyper-expression of vaccine antigens or therapeutic proteins in transgenic chloroplasts (leaves) or chromoplasts (fruits/roots) facilitates efficient oral delivery. Ability of chloroplasts to correctly fold human blood proteins with proper disulfide bridges (human serum albumin or interferons) or presence of chaperones in chloroplasts to facilitate assembly of complex multi-subunit proteins or their prokaryotic nature to express native bacterial genes (up to 46.1% total leaf protein) are attractive features for therapeutic protein production. Purification of therapeutic proteins has been achieved using novel purification strategies that do not require expensive column chromatography. (c) 2004 Elsevier Ltd. All rights reserved.

    Journal Title

    Vaccine

    Volume

    23

    Issue/Number

    15

    Publication Date

    1-1-2005

    Document Type

    Article; Proceedings Paper

    Language

    English

    First Page

    1779

    Last Page

    1783

    WOS Identifier

    WOS:000227769600005

    ISSN

    0264-410X

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