Breakthrough in chloroplast genetic engineering of agronomically important crops

    Authors

    H. Daniell; S. Kumar;N. Dufourmantel

    Comments

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    Abbreviated Journal Title

    Trends Biotechnol.

    Keywords

    DAUCUS-CAROTA L.; TOBACCO CHLOROPLASTS; PLASTID TRANSFORMATION; SALT; TOLERANCE; TRANSGENIC CHLOROPLASTS; TRANSPLASTOMIC TOBACCO; STABLE; TRANSFORMATION; MATERNAL INHERITANCE; SELECTABLE MARKER; POLLEN; DISPERSAL; Biotechnology & Applied Microbiology

    Abstract

    Chloroplast genetic engineering offers several unique advantages, including high-level transgene expression, multi-gene engineering in a single transformation event and transgene containment by maternal inheritance, as well as a lack of gene silencing, position and pleiotropic effects and undesirable foreign DNA. More than 40 trainsgenes have been stably integrated and expressed using the tobacco chloroplast genome to confer desired agronomic traits or express high levels of vaccine antigens and biopharmaceuticals. Despite such significant progress, this technology has not been extended to major crops. However, highly efficient soybean, carrot and cotton plastid transformation has recently been accomplished through somatic embryogenesis using species-specific chloroplast vectors. This review focuses on recent exciting developments in this field and offers directions for further research and development.

    Journal Title

    Trends in Biotechnology

    Volume

    23

    Issue/Number

    5

    Publication Date

    1-1-2005

    Document Type

    Editorial Material

    Language

    English

    First Page

    238

    Last Page

    245

    WOS Identifier

    WOS:000229466000006

    ISSN

    0167-7799

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