Title

Breakthrough in chloroplast genetic engineering of agronomically important crops

Authors

Authors

H. Daniell; S. Kumar;N. Dufourmantel

Comments

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Abbreviated Journal Title

Trends Biotechnol.

Keywords

DAUCUS-CAROTA L.; TOBACCO CHLOROPLASTS; PLASTID TRANSFORMATION; SALT; TOLERANCE; TRANSGENIC CHLOROPLASTS; TRANSPLASTOMIC TOBACCO; STABLE; TRANSFORMATION; MATERNAL INHERITANCE; SELECTABLE MARKER; POLLEN; DISPERSAL; Biotechnology & Applied Microbiology

Abstract

Chloroplast genetic engineering offers several unique advantages, including high-level transgene expression, multi-gene engineering in a single transformation event and transgene containment by maternal inheritance, as well as a lack of gene silencing, position and pleiotropic effects and undesirable foreign DNA. More than 40 trainsgenes have been stably integrated and expressed using the tobacco chloroplast genome to confer desired agronomic traits or express high levels of vaccine antigens and biopharmaceuticals. Despite such significant progress, this technology has not been extended to major crops. However, highly efficient soybean, carrot and cotton plastid transformation has recently been accomplished through somatic embryogenesis using species-specific chloroplast vectors. This review focuses on recent exciting developments in this field and offers directions for further research and development.

Journal Title

Trends in Biotechnology

Volume

23

Issue/Number

5

Publication Date

1-1-2005

Document Type

Editorial Material

Language

English

First Page

238

Last Page

245

WOS Identifier

WOS:000229466000006

ISSN

0167-7799

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