Title

Adult rat spinal cord culture on an organosilane surface in a novel serum-free medium

Authors

Authors

M. Das; N. Bhargava; C. Gregory; L. Riedel; P. Molnar;J. J. Hickman

Comments

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Abbreviated Journal Title

In Vitro Cell. Dev. Biol.-Anim.

Keywords

adult rat spinal cord; cell culture; motoneuron; silane surface; serum-free medium; electrophysiology; FIBROBLAST-GROWTH-FACTOR; CENTRAL-NERVOUS-SYSTEM; LONG-TERM SURVIVAL; IN-VITRO; HIPPOCAMPAL-NEURONS; FACIAL-NERVE; NEUROTROPHIC FACTORS; DEFINED SYSTEM; RETINOIC ACID; MOTOR-NEURONS; Cell Biology; Developmental Biology

Abstract

In this study, we have documented by morphological analysis, immunocytochemistry, and electrophysiology thedeveloptuent of a culture system that promotes the growth and long-term survival of dissociated adult rat spinal cord neurons. This system comprises a patternable, nonbiological, cell growth-promoting organosilane substrate coated oil a glass surface and all empirically derived novel serum-free medium, supplemented with specific growth factors (acidic fibroblast growth factor, heparin sulfate, neurotrophin-3. brain-derived neurotrophic factor, glial-derived neurotrophic factor, cardiotrophin-1, and vitronectin). Neurons were characterized by immunoreactivity for neurofilament 150, neuron-specific enolase, Islet-1 antibodies, electrophysiology, and the Cultures were maintained for 4-6 wk. This culture system could be a useful tool for the study of adult mammalian spinal neurons in a functional ill vitro system.

Journal Title

In Vitro Cellular & Developmental Biology-Animal

Volume

41

Issue/Number

10

Publication Date

1-1-2005

Document Type

Article

Language

English

First Page

343

Last Page

348

WOS Identifier

WOS:000236070000006

ISSN

1071-2690

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