Evaluation of two lanthanide complexes for qualitative and quantitative analysis of target proteins via partial least squares analysis

    Authors

    H. Goicoechea; B. C. Roy; M. Santos; A. D. Campiglia;S. Mallik

    Comments

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    Abbreviated Journal Title

    Anal. Biochem.

    Keywords

    luminescence; lanthanide ions; carbonic anhydrase; gamma-globulin; human; serum albumin; partial least squares regression; proteins; HUMAN SERUM-ALBUMIN; SPECTROPHOTOMETRIC DETERMINATION; GAMMA-GLOBULIN; CALIBRATION; PROBE; SPECTROSCOPY; IONS; Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Analytical

    Abstract

    Two lanthanide complexes, namely 5-aminosalicylic acid ethylenediaminetetraacetate europium(III) (5As-EDTA-Eu3+) and 4-aminosalicylic acid ethylenediaminetetraacetate terbium(III), were evaluated for the analysis of carbonic anhydrase, human serum albumin (HSA), and gamma-globulin. Quantitative analysis is based on their luminescence enhancement upon protein binding and qualitative analysis on their lifetime capability to recognize the binding protein. Analytical figures of merit are presented for the three proteins. The limits of detection with 5As-EDTA-Eu3+ are at the parts per billion level. Partial least square regression analysis is used to determine HSA and gamma-globulin in binary mixtures without previous separation at the concentration ranges typically found in clinical tests of human blood serum. (C) 2004 Elsevier Inc. All rights reserved.

    Journal Title

    Analytical Biochemistry

    Volume

    336

    Issue/Number

    1

    Publication Date

    1-1-2005

    Document Type

    Article

    Language

    English

    First Page

    64

    Last Page

    74

    WOS Identifier

    WOS:000225918900009

    ISSN

    0003-2697

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