Title

Evaluation of two lanthanide complexes for qualitative and quantitative analysis of target proteins via partial least squares analysis

Authors

Authors

H. Goicoechea; B. C. Roy; M. Santos; A. D. Campiglia;S. Mallik

Comments

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Abbreviated Journal Title

Anal. Biochem.

Keywords

luminescence; lanthanide ions; carbonic anhydrase; gamma-globulin; human; serum albumin; partial least squares regression; proteins; HUMAN SERUM-ALBUMIN; SPECTROPHOTOMETRIC DETERMINATION; GAMMA-GLOBULIN; CALIBRATION; PROBE; SPECTROSCOPY; IONS; Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Analytical

Abstract

Two lanthanide complexes, namely 5-aminosalicylic acid ethylenediaminetetraacetate europium(III) (5As-EDTA-Eu3+) and 4-aminosalicylic acid ethylenediaminetetraacetate terbium(III), were evaluated for the analysis of carbonic anhydrase, human serum albumin (HSA), and gamma-globulin. Quantitative analysis is based on their luminescence enhancement upon protein binding and qualitative analysis on their lifetime capability to recognize the binding protein. Analytical figures of merit are presented for the three proteins. The limits of detection with 5As-EDTA-Eu3+ are at the parts per billion level. Partial least square regression analysis is used to determine HSA and gamma-globulin in binary mixtures without previous separation at the concentration ranges typically found in clinical tests of human blood serum. (C) 2004 Elsevier Inc. All rights reserved.

Journal Title

Analytical Biochemistry

Volume

336

Issue/Number

1

Publication Date

1-1-2005

Document Type

Article

Language

English

First Page

64

Last Page

74

WOS Identifier

WOS:000225918900009

ISSN

0003-2697

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