Controllable self-assembly of nanoparticles for specific delivery of multiple therapeutic molecules to cancer cells using RNA nanotechnology

    Authors

    A. Khaled; S. C. Guo; F. Li;P. X. Guo

    Comments

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    Abbreviated Journal Title

    Nano Lett.

    Keywords

    DNA-PACKAGING PRNA; PHAGE PHI 29; BACTERIOPHAGE-PHI-29 DNA; CHEMICAL-MODIFICATION; SEQUENCE REQUIREMENT; INTERFERING RNAS; MAMMALIAN-CELLS; POTENTIAL PARTS; IN-VIVO; INHIBITION; Chemistry, Multidisciplinary; Chemistry, Physical; Nanoscience &; Nanotechnology; Materials Science, Multidisciplinary; Physics, Applied; Physics, Condensed Matter

    Abstract

    By utilizing RNA nanotechnology, we engineered both therapeutic siRNA and a receptor-binding RNA aptamer into individual pRNAs of phi29's motor. The RNA building block harboring siRNA or other therapeutic molecules was fabricated subsequently into a trimer through the interaction of engineered right and left interlocking RNA loops. The incubation of the protein-free nanoscale particles containing the receptor-binding aptamer or other ligands resulted in the binding and co-entry of the trivalent therapeutic particles into cells, subsequently modulating the apoptosis of cancer cells and leukemia model lymphocytes in cell culture and animal trials. The use of such antigenicity-free 20-40 nm particles holds promise for the repeated long-term treatment of chronic diseases.

    Journal Title

    Nano Letters

    Volume

    5

    Issue/Number

    9

    Publication Date

    1-1-2005

    Document Type

    Article

    Language

    English

    First Page

    1797

    Last Page

    1808

    WOS Identifier

    WOS:000231945500031

    ISSN

    1530-6984

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