Title

Controllable self-assembly of nanoparticles for specific delivery of multiple therapeutic molecules to cancer cells using RNA nanotechnology

Authors

Authors

A. Khaled; S. C. Guo; F. Li;P. X. Guo

Comments

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Abbreviated Journal Title

Nano Lett.

Keywords

DNA-PACKAGING PRNA; PHAGE PHI 29; BACTERIOPHAGE-PHI-29 DNA; CHEMICAL-MODIFICATION; SEQUENCE REQUIREMENT; INTERFERING RNAS; MAMMALIAN-CELLS; POTENTIAL PARTS; IN-VIVO; INHIBITION; Chemistry, Multidisciplinary; Chemistry, Physical; Nanoscience &; Nanotechnology; Materials Science, Multidisciplinary; Physics, Applied; Physics, Condensed Matter

Abstract

By utilizing RNA nanotechnology, we engineered both therapeutic siRNA and a receptor-binding RNA aptamer into individual pRNAs of phi29's motor. The RNA building block harboring siRNA or other therapeutic molecules was fabricated subsequently into a trimer through the interaction of engineered right and left interlocking RNA loops. The incubation of the protein-free nanoscale particles containing the receptor-binding aptamer or other ligands resulted in the binding and co-entry of the trivalent therapeutic particles into cells, subsequently modulating the apoptosis of cancer cells and leukemia model lymphocytes in cell culture and animal trials. The use of such antigenicity-free 20-40 nm particles holds promise for the repeated long-term treatment of chronic diseases.

Journal Title

Nano Letters

Volume

5

Issue/Number

9

Publication Date

1-1-2005

Document Type

Article

Language

English

First Page

1797

Last Page

1808

WOS Identifier

WOS:000231945500031

ISSN

1530-6984

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