Solid-supported synthesis of polymerizable lanthanide-ion chelating lipids for protein detection

    Authors

    S. Nadi; M. Santos; M. K. Haldar; B. C. Roy; S. Mallik;A. D. Campiglia

    Comments

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    Abbreviated Journal Title

    Inorg. Chem.

    Keywords

    HUMAN CARBONIC-ANHYDRASE; PHASE SYNTHESIS; HIGHLY LUMINESCENT; FIBEROPTIC PROBE; LIPOSOMES; COMPLEXES; SPECTROSCOPY; TRANSFECTION; STABILITY; DELIVERY; Chemistry, Inorganic & Nuclear

    Abstract

    Usually, lipids are synthesized employing solution-phase organic synthesis techniques. Though successful, the purifications can be difficult to accomplish due to the amphiphilic nature of the molecules. Herein, we demonstrate the advantages of a solid-phase approach for preparing a variety of metal-chelating lipids. A number of saturated and polymerizable metal-chelating lipids were prepared using this methodology. This approach requires one chromatographic purification after cleaving the lipids from the solid support. We also demonstrate that the resulting polymerized liposomes (containing Eu3+) possess the appropriate luminescence properties for the qualitative and quantitative determination of proteins.

    Journal Title

    Inorganic Chemistry

    Volume

    44

    Issue/Number

    7

    Publication Date

    1-1-2005

    Document Type

    Article

    Language

    English

    First Page

    2234

    Last Page

    2244

    WOS Identifier

    WOS:000228054200018

    ISSN

    0020-1669

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