Transient alterations in granule cell proliferation, apoptosis and migration in postnatal developing cerebellum of CRMP1(-/-) mice

    Authors

    E. Charrier; B. Mosinger; C. Meissirel; M. Aguera; V. Rogemond; S. Reibel; P. Salin; N. Chounlamountri; V. Perrot; M. F. Belin; Y. Goshima; J. Honnorat; N. Thomasset;P. Kolattukudy

    Comments

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    Abbreviated Journal Title

    Genes Cells

    Keywords

    RESPONSE MEDIATOR PROTEIN-2; ALZHEIMERS-DISEASE; NEURONAL MIGRATION; NERVOUS-SYSTEM; AXON GUIDANCE; COLLAPSIN; INVOLVEMENT; EXPRESSION; BRAIN; IDENTIFICATION; Cell Biology; Genetics & Heredity

    Abstract

    Collapsin response mediator proteins (CRMPs) consist of five homologous cytosolic proteins that participate in signal transduction involved in a variety of physiological events. CRMP1 is highly expressed during brain development; however, its functions remains unclear. To gain insight into its function, we generated CRMP1(-/-) mice with a knock-in LacZ gene. No gross anatomical changes or behavioral alterations were observed. Expression of CRMP1 was examined by the expression of the knocked-in LacZ gene, in situ hybridization with riboprobes and by imunohistochemistry. CRMP1 was found to be highly expressed in the developing the cerebellum, olfactory bulbs, hypothalamus and retina. In adults, expression level was high in the olfactory bulbs and hippocampus but very low in the retina and cerebellum and undetectable in hypothalamus. To study potential roles of CRMP1, we focused on cerebellum development. CRMP1(-/-) mice showed a decrease in the number of granule cells migrating out of explants of developing cerebellum, as did treatment of the explants from normal mice with anti-CRMP1 specific antibodies. CRMP1(-/-) mice showed a decrease in granule cell proliferation and apoptosis in external granule cell layers in vivo. Adult cerebellum of CRMP1(-/-) did not show any abnormalities.

    Journal Title

    Genes to Cells

    Volume

    11

    Issue/Number

    12

    Publication Date

    1-1-2006

    Document Type

    Article

    Language

    English

    First Page

    1337

    Last Page

    1352

    WOS Identifier

    WOS:000242195800002

    ISSN

    1356-9597

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