Title
A defined system to allow skeletal muscle differentiation and subsequent integration with silicon microstructures
Abbreviated Journal Title
Biomaterials
Keywords
hybrid devices; defined system; serum-free; myotubes; MEMS; NERVE GROWTH-FACTOR; HIPPOCAMPAL-NEURONS; NEUROTROPHIC FACTOR; CELL-ADHESION; IN-VITRO; PROLIFERATION; SURVIVAL; CULTURE; SURFACE; CARDIOTROPHIN-1; Engineering, Biomedical; Materials Science, Biomaterials
Abstract
This work documents the development of an in vitro cell culture model consisting of a novel serum-free medium and a non-biological growth substrate, N-1[3 (trimethoxysilyl) propyl] diethylenetriamine (DETA), to enable functional myotube integration with cantilevers fabricated using MEMS technology. This newly developed, defined in vitro model was used to study the differentiation of fetal rat skeletal muscle and it promoted the formation of myotubes from the dissociated rat fetal muscle cells. The myotubes were characterized by morphological analysis, immunocytochemistry and electrophysiology. Further, it was demonstrated that when the dissociated muscle cells were plated on fabricated microcantilevers, the muscle cells aligned along the major axis of the cantilever and formed robust myotubes. This novel system could not only find applications in skeletal muscle differentiation and biocompatibility studies but also in bioartificial muscle engineering, hybrid actuation system development, biorobotics and for a better understanding of myopathies and neuromuscular disorders. (c) 2006 Elsevier Ltd. All rights reserved.
Journal Title
Biomaterials
Volume
27
Issue/Number
24
Publication Date
1-1-2006
Document Type
Article
Language
English
First Page
4374
Last Page
4380
WOS Identifier
ISSN
0142-9612
Recommended Citation
"A defined system to allow skeletal muscle differentiation and subsequent integration with silicon microstructures" (2006). Faculty Bibliography 2000s. 6061.
https://stars.library.ucf.edu/facultybib2000/6061
Comments
Authors: contact us about adding a copy of your work at STARS@ucf.edu