Title
Amyloid precursor protein regulates differentiation of human neural stem cells
Abbreviated Journal Title
Stem Cells Dev.
Keywords
ADULT SUBVENTRICULAR ZONE; ALZHEIMERS-DISEASE; BINDING PROTEIN; HUMAN; BRAIN; BETA; MICE; GENE; MIGRATION; APOPTOSIS; APP; Cell & Tissue Engineering; Hematology; Medicine, Research &; Experimental; Transplantation
Abstract
Although amyloid beta(A beta) deposition has been a hallmark of Alzheimer's disease (AD), the absence of a phenotype in the beta amyloid precursor protein (APP) knockout mouse, tends to detract our attention away from the physiological functions of APP. Although much attention has been focused on the neurotoxicity of A beta, many studies suggest the involvement of APP in neuroplasticity. We found that secreted amyloid precursor protein ( sAPP) increased the differentiation of human neural stem cells (hNSCs) in vitro, while an antibody-recognizing APP dose-dependently inhibited these activities. With a high dose of sAPP treatment or wild-type APP gene transfection, hNSCs were differentiated into astrocytes rather than neurons. In vivo, hNSCs transplanted into APP-transgenic mouse brain exhibited glial differentiation rather than neural differentiation. Our results suggest that APP regulates neural stem cell biology in the adult brain, and that altered APP metabolism in Down syndrome or AD may have implications for the pathophysiology of these diseases.
Journal Title
Stem Cells and Development
Volume
15
Issue/Number
3
Publication Date
1-1-2006
Document Type
Article
Language
English
First Page
381
Last Page
389
WOS Identifier
ISSN
1547-3287
Recommended Citation
"Amyloid precursor protein regulates differentiation of human neural stem cells" (2006). Faculty Bibliography 2000s. 6325.
https://stars.library.ucf.edu/facultybib2000/6325
Comments
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