Interference of EGFP RNA in human NT-2/D1 cell lines using human u6 promoter-based siRNA PCR products

    Authors

    Y. D. Kwak;K. Sugaya

    Comments

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    Abbreviated Journal Title

    Biotechnol. Bioprocess Eng.

    Keywords

    gene silencing; small interference RNA; PCR; human teratocarcinoma; NT-2/D1 cells; DOUBLE-STRANDED-RNA; MAMMALIAN-CELLS; GENE-EXPRESSION; SUPPRESSION; PROTEINS; VECTOR; Biotechnology & Applied Microbiology

    Abstract

    RNA interference (RNAi), a process of sequence-specific gene suppression, has been known as a natural gene regulatory mechanism in a wide range of lower organisms. Recently, we have reported that a transfection of human U6 promoter (hU6) driven hairpin small-interference RNA (siRNA) plasmid specifically knocks down the target gene by posttranscriptional gene silencing in mammalian cells. Here we report that transfection of polymerase chain reaction (PCR) products, containing human U6 promoter with hairpin siRNA, knocks down the target gene expression in human teratocarcinoma NT-2/D1 cells. Moreover, we showed 3' end termination sequence, 5 Ts, is not critical elements for knocking down in PCR-based siRNA system. Therefore, the PCR-based siRNA system is a promising tool not only for the screening but also to temporally regulate gene expression in the human progenitor cells.

    Journal Title

    Biotechnology and Bioprocess Engineering

    Volume

    11

    Issue/Number

    3

    Publication Date

    1-1-2006

    Document Type

    Article

    Language

    English

    First Page

    273

    Last Page

    276

    WOS Identifier

    WOS:000238768600016

    ISSN

    1226-8372

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