Title
Plastid transformation in the monocotyledonous cereal crop, rice (Oryza sativa) and transmission of transgenes to their progeny
Abbreviated Journal Title
Mol. Cells
Keywords
cereal crop; chloroplast genome; monocotyledonous; plant species; plastid transformation; rice; GREEN FLUORESCENT PROTEIN; TRANSFER-RNA GENES; CHLOROPLAST GENOME; TOBACCO CHLOROPLASTS; HIGHER-PLANTS; HERBICIDE RESISTANCE; PROTECTIVE; ANTIGEN; EXPRESSION; VACCINE; MARKER; Biochemistry & Molecular Biology; Cell Biology
Abstract
The plastid transformation approach offers a number of unique advantages, including high-level transgene expression, multi-gene engineering, transgene containment, and a lack of gene silencing and position effects. The extension of plastid transformation technology to monocotyledonous cereal crops, including rice, bears great promise for the improvement of agronomic traits, and the efficient production of pharmaceutical or nutritional enhancement. Here, we report a promising step towards stable plastid transformation in rice. We produced fertile transplastomic rice plants and demonstrated transmission of the plastid-expressed green fluorescent protein (GFP) and aminoglycoside 3'-adenylyltransferase genes to the progeny of these plants. Transgenic chloroplasts were determined to have stably expressed the GFP, which was confirmed by both confocal microscopy and Western blot analyses. Although the produced rice plastid transformants were found to be heteroplastomic, and the transformation efficiency requires further improvement, this study has established a variety of parameters for the use of plastid transformation technology in cereal crops.
Journal Title
Molecules and Cells
Volume
21
Issue/Number
3
Publication Date
1-1-2006
Document Type
Article
Language
English
First Page
401
Last Page
410
WOS Identifier
ISSN
1016-8478
Recommended Citation
"Plastid transformation in the monocotyledonous cereal crop, rice (Oryza sativa) and transmission of transgenes to their progeny" (2006). Faculty Bibliography 2000s. 6339.
https://stars.library.ucf.edu/facultybib2000/6339
Comments
Authors: contact us about adding a copy of your work at STARS@ucf.edu