Plastid transformation in the monocotyledonous cereal crop, rice (Oryza sativa) and transmission of transgenes to their progeny

    Authors

    S. M. Lee; K. S. Kang; H. Chung; S. H. Yoo; X. M. Xu; S. B. Lee; J. J. Cheong; H. Daniell;M. Kim

    Comments

    Authors: contact us about adding a copy of your work at STARS@ucf.edu

    Abbreviated Journal Title

    Mol. Cells

    Keywords

    cereal crop; chloroplast genome; monocotyledonous; plant species; plastid transformation; rice; GREEN FLUORESCENT PROTEIN; TRANSFER-RNA GENES; CHLOROPLAST GENOME; TOBACCO CHLOROPLASTS; HIGHER-PLANTS; HERBICIDE RESISTANCE; PROTECTIVE; ANTIGEN; EXPRESSION; VACCINE; MARKER; Biochemistry & Molecular Biology; Cell Biology

    Abstract

    The plastid transformation approach offers a number of unique advantages, including high-level transgene expression, multi-gene engineering, transgene containment, and a lack of gene silencing and position effects. The extension of plastid transformation technology to monocotyledonous cereal crops, including rice, bears great promise for the improvement of agronomic traits, and the efficient production of pharmaceutical or nutritional enhancement. Here, we report a promising step towards stable plastid transformation in rice. We produced fertile transplastomic rice plants and demonstrated transmission of the plastid-expressed green fluorescent protein (GFP) and aminoglycoside 3'-adenylyltransferase genes to the progeny of these plants. Transgenic chloroplasts were determined to have stably expressed the GFP, which was confirmed by both confocal microscopy and Western blot analyses. Although the produced rice plastid transformants were found to be heteroplastomic, and the transformation efficiency requires further improvement, this study has established a variety of parameters for the use of plastid transformation technology in cereal crops.

    Journal Title

    Molecules and Cells

    Volume

    21

    Issue/Number

    3

    Publication Date

    1-1-2006

    Document Type

    Article

    Language

    English

    First Page

    401

    Last Page

    410

    WOS Identifier

    WOS:000238843800012

    ISSN

    1016-8478

    Share

    COinS