Expression of prostasin and protease nexin-1 in rhesus monkey (Macaca mulatta) endometrium and placenta during early pregnancy

    Authors

    H. Y. Lin; H. Zhang; Q. Yang; H. X. Wang; H. M. Wang; K. X. Chai; L. M. Chen;C. Zhu

    Comments

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    Abbreviated Journal Title

    J. Histochem. Cytochem.

    Keywords

    prostasin; protease nexin-1; endometrium; placenta; rhesus monkey; pregnancy; EPITHELIAL SODIUM-CHANNEL; SERINE-PROTEASE; GROWTH-FACTOR; PLASMINOGEN-ACTIVATOR; EMBRYO IMPLANTATION; MOUSE ENDOMETRIUM; LOCALIZATION; PROTEINASE; INHIBITOR; SECRETION; Cell Biology

    Abstract

    Serine proteases have been documented to play key roles in uterine matrix turnover and trophoblastic invasion during implantation. Roles of prostasin serine protease in these processes, however, are currently unclear. The present study was first conducted to investigate the colocalization of prostasin and its cognate serpin, protease nexin-1 (PN-1), in rhesus monkey endometrium and placenta on days 12, 18, and 26 of pregnancy by using in situ hybridization (ISH) and immunohistochemistry. With ISH, expression of prostasin mRNA was intensely localized in the glandular epithelium on days 12 and 18 and in the placental villi, trophoblastic column, trophoblastic shell, and fetal-maternal border on days 18 and 26. With the progress of pregnancy, expression level in the glandular epithelium was significantly decreased, and the accumulation in the placental compartments was further increased. In addition, the stroma and arterioles exhibited modest levels of prostasin signals. However, expression level of PN-1 in these compartments on adjacent sections in the three stages of early pregnancy was weak or below the level of detection. Prostasin protein expression in the endometrium was found to be consistent with the distribution patterns revealed in the ISH experiments. It may be suggested from these results that prostasin is involved in endometrial epithelial morphology establishment, tissue remodeling, and trophoblastic invasion during early pregnancy. The cognate serpin PN-1 was not coordinately expressed along with prostasin, creating a tissue environment favorable for proteolytic activities of prostasin during early pregnancy events.

    Journal Title

    Journal of Histochemistry & Cytochemistry

    Volume

    54

    Issue/Number

    10

    Publication Date

    1-1-2006

    Document Type

    Article

    Language

    English

    First Page

    1139

    Last Page

    1147

    WOS Identifier

    WOS:000240672800008

    ISSN

    0022-1554

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