Identification of a diacylglycerol acyltransferase gene involved in accumulation of triacylglycerol in Mycobacterium tuberculosis under stress

    Authors

    T. D. Sirakova; V. S. Dubey; C. Deb; J. Daniel; T. A. Korotkova; B. Abomoelak;P. E. Kolattukudy

    Comments

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    Abbreviated Journal Title

    Microbiology-(UK)

    Keywords

    NITRIC-OXIDE; FATTY-ACIDS; BOVIS BCG; EXPRESSION; INFECTION; SYNTHASE; PERSISTENCE; PHAGOSOMES; Microbiology

    Abstract

    Mycobacterium tuberculosis under stress stores triacylglycerol (TG). There are 15 genes in M. tuberculosis that belong to a novel family of TG synthase genes (tgs), but it is not known which of them is responsible for this accumulation of TG. In this paper, it is reported that M. tuberculosis H37Rv accumulated TG under acidic, static or hypoxic growth conditions, or upon treatment with NO, whereas TG accumulation was drastically reduced in the tgs1 (Rv3130c) disrupted mutant. Complementation with tgs1 restored this TG accumulation. C-26 was a major fatty acid in this TG, indicating that the TGS1 gene product uses C-26 fatty acid, which is known to be produced by the mycobacterial fatty acid synthase. TGS1 expressed in Escherichia coli preferred C-26:0-CoA for TG synthesis. If TG storage is needed for the long-term survival of M. tuberculosis under dormant conditions, the tgs1 product could be a suitable target for antilatency drugs.

    Journal Title

    Microbiology-Sgm

    Volume

    152

    Publication Date

    1-1-2006

    Document Type

    Article

    Language

    English

    First Page

    2717

    Last Page

    2725

    WOS Identifier

    WOS:000240693600021

    ISSN

    1350-0872

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