A water-soluble diaminostilbene derivative as a two-photon fluorescent probe

    Authors

    S. Yao; K. J. Schafer-Hales; I. Cohanoschi; F. E. Hernandez;K. D. Belfield

    Comments

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    Abbreviated Journal Title

    Synlett

    Keywords

    two-photon; fluorescent probe; PAMAM : hydrophilic; Z-scan; two-photon; microscopy; BIS(STYRYL)BENZENE DERIVATIVES; DIPYRRYLMETHENE-BF2 DYES; COOPERATIVE; ENHANCEMENT; ABSORBING CHROMOPHORES; SUBSTITUTED STILBENES; ABSORPTION; MICROSCOPY; DONOR; DENDRIMERS; FEMTOSECOND; Chemistry, Organic

    Abstract

    A hydrophilic trans-4,4'-diaminostilbene derivative was prepared by the introduction of amideamino peripheral substituents. Very high aqueous solubility was achieved ( > 15 mM), making it possible as a fluorescent probe for biological imaging. The relatively straightforward synthetic methodology has great potential to transform even large hydrophobic two-photon absorbing chromophores into hydrophilic derivatives. The fluorescence quantum yield of 0.27 for the diammostilbene in water is sufficiently high for use as a fluorescent probe. In addition, two-photon fluorescence images of NT2 cells stained by this probe were also obtained.

    Journal Title

    Synlett

    Issue/Number

    12

    Publication Date

    1-1-2006

    Document Type

    Article

    Language

    English

    First Page

    1863

    Last Page

    1866

    WOS Identifier

    WOS:000240009100011

    ISSN

    0936-5214

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