Title

Embryonic motoneuron-skeletal muscle co-culture in a defined system

Authors

Authors

M. Das; J. W. Rumsey; C. A. Gregory; N. Bhargava; J. F. Kang; P. Molnar; L. Riedel; X. Guo;J. J. Hickman

Comments

Authors: contact us about adding a copy of your work at STARS@ucf.edu

Abbreviated Journal Title

Neuroscience

Keywords

defined system; neuromuscular junction; self-assembled monolayer; motoneuron; serum-free medium; skeletal muscle; SERUM-FREE MEDIUM; RAT SPINAL-CORD; NEUROMUSCULAR-JUNCTION; IN-VITRO; ACETYLCHOLINE-RECEPTOR; ORGANOSILANE SURFACE; HIPPOCAMPAL-NEURONS; CULTURE; GROWTH; CELLS; Neurosciences

Abstract

This paper describes a significant biotechnological advancement by creating a minimalist serum-free defined system to co-culture rat mammalian nerve and muscle cells in order to form functional neuromuscular junctions. To date, all the known in vitro nerve and muscle co-culture models use serum containing media; and while functional neuromuscular junctions (NMJ) are described, they failed to detail or quantify the minimum factors needed to recreate the NMJ in vitro. In this work, we demonstrate the development of a defined motoneuron and muscle co-culture system resulting in the formation of NMJs including: 1) a new culture technique, 2) a novel serum-free medium formulation and 3) a synthetic self-assembled monolayer (SAM) substrate N-1 [3-(trimethoxysilyi) propyl] diethylenetriamine (DETA). We characterized the culture by morphology, immunocytochemistry, electrophysiology and videography. This model system provides a better understanding of the minimal growth factor and substrate interactions necessary for NMJ formation and provides a basic system that can be utilized for nerve-muscle tissue engineering, regenerative medicine and development of limb prosthetics. (C) 2007 IBRO. Published by Elsevier Ltd. All rights reserved.

Journal Title

Neuroscience

Volume

146

Issue/Number

2

Publication Date

1-1-2007

Document Type

Article

Language

English

First Page

481

Last Page

488

WOS Identifier

WOS:000246535000001

ISSN

0306-4522

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