Title

Differentiation of skeletal muscle and integration of myotubes with silicon microstructures using serum-free medium and a synthetic silane substrate

Authors

Authors

M. Das; K. Wilson; P. Molnar;J. J. Hickman

Comments

Authors: contact us about adding a copy of your work at STARS@ucf.edu

Abbreviated Journal Title

Nat. Protoc.

Keywords

ORGANOSILANE SURFACE; DEFINED SYSTEM; IN-VITRO; RAT; NETWORKS; CULTURE; Biochemical Research Methods

Abstract

This protocol describes a cell culture model to study the differentiation of fetal rat skeletal muscle cells. The model uses serum-free medium, a nonbiological substrate N-1[3(trimethoxysilyl)propyl] diethylenetriamine ( DETA) and fabricated microcantilevers to promote the differentiation of dissociated rat myocytes into robust myotubes. In this protocol, we also describe how to characterize the myotubes on the basis of morphology, immunocytochemistry and electrophysiology. Here, four major techniques are employed: fabrication of cantilevers, surface modification of the glass and cantilever substrates with a DETA SAM, a serum-free medium and refined culture techniques. This culture system has potential applications in biocompatibility studies, bioartificial muscle engineering, skeletal muscle differentiation studies and for better understanding of myopathies and neuromuscular disorders. The model can be established in 26-33 d.

Journal Title

Nature Protocols

Volume

2

Issue/Number

7

Publication Date

1-1-2007

Document Type

Article

Language

English

First Page

1795

Last Page

1801

WOS Identifier

WOS:000253139200026

ISSN

1754-2189

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