Title

Isolation and characterization of microsatellite loci from the endangered highlands scrub hypericum (Hypericum cumulicola)

Authors

Authors

C. E. Edwards; M. Arakaki; P. F. Quintana-Ascencio; D. E. Soltis;P. S. Soltis

Comments

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Abbreviated Journal Title

Mol. Ecol. Notes

Keywords

highlands scrub hypericum; Hypericaceae; Hypericum cumulicola; microsatellite; Biochemistry & Molecular Biology; Ecology; Evolutionary Biology

Abstract

We report the isolation of 19 primer pairs for amplification of polymorphic microsatellite loci for Hypericum cumulicola. These markers were evaluated in 24 individuals from one population; two to four alleles were detected per locus, and observed heterozygosity ranged from 0 to 0.5. Two loci demonstrated significant heterozygote deficiencies, possibly due to null alleles, and significant linkage disequilibrium was found between six pairs of loci. The remaining microsatellite loci will help determine if genetic differentiation is responsible for life-history differences between natural and anthropogenically disturbed populations of H. cumulicola.

Journal Title

Molecular Ecology Notes

Volume

7

Issue/Number

6

Publication Date

1-1-2007

Document Type

Article

Language

English

First Page

1135

Last Page

1137

WOS Identifier

WOS:000250647700060

ISSN

1471-8278

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