Isolation and characterization of microsatellite loci from the endangered highlands scrub hypericum (Hypericum cumulicola)

    Authors

    C. E. Edwards; M. Arakaki; P. F. Quintana-Ascencio; D. E. Soltis;P. S. Soltis

    Comments

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    Abbreviated Journal Title

    Mol. Ecol. Notes

    Keywords

    highlands scrub hypericum; Hypericaceae; Hypericum cumulicola; microsatellite; Biochemistry & Molecular Biology; Ecology; Evolutionary Biology

    Abstract

    We report the isolation of 19 primer pairs for amplification of polymorphic microsatellite loci for Hypericum cumulicola. These markers were evaluated in 24 individuals from one population; two to four alleles were detected per locus, and observed heterozygosity ranged from 0 to 0.5. Two loci demonstrated significant heterozygote deficiencies, possibly due to null alleles, and significant linkage disequilibrium was found between six pairs of loci. The remaining microsatellite loci will help determine if genetic differentiation is responsible for life-history differences between natural and anthropogenically disturbed populations of H. cumulicola.

    Journal Title

    Molecular Ecology Notes

    Volume

    7

    Issue/Number

    6

    Publication Date

    1-1-2007

    Document Type

    Article

    Language

    English

    First Page

    1135

    Last Page

    1137

    WOS Identifier

    WOS:000250647700060

    ISSN

    1471-8278

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