mRNA profiling for body fluid identification by multiplex quantitative RT-PCR

    Authors

    J. Juusola;J. Ballantyne

    Comments

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    Abbreviated Journal Title

    J. Forensic Sci.

    Keywords

    forensic science; RNA; mRNA profiling; body fluid identification; blood; identification; saliva identification; semen identification; menstrual; blood identification; multiplex real-time PCR; DELTA-AMINOLEVULINATE SYNTHASE; GENE; Medicine, Legal

    Abstract

    An alternative approach to conventional protein-based body fluid identification is gene expression profiling analysis. In the present work, we report the development of sensitive and robust multiplex quantitative reverse transcriptase-PCR assays for the identification of blood, saliva, semen, and menstrual blood. Each body fluid assay comprises a triplex system that detects transcripts from two body fluid-specific genes and a housekeeping gene GAPDH. The body fluid-specific genes include erythroid delta-aminolevulinate synthase (ALAS2) and beta-spectrin (SPTB) for blood, statherin (STATH) and histatin 3 (HTN3) for saliva, protamine 1 (PRM1) and protamine 2 (PRM2) for semen, and matrix metalloproteinase 7 (MMP7) and matrix metalloproteinase 10 (MMP10) for menstrual blood. Normalization of both body fluid-specific genes to the housekeeping gene by means of appropriate cycle threshold metrics ensures the high specificity of each assay for its cognate body fluid.

    Journal Title

    Journal of Forensic Sciences

    Volume

    52

    Issue/Number

    6

    Publication Date

    1-1-2007

    Document Type

    Article; Proceedings Paper

    Language

    English

    First Page

    1252

    Last Page

    1262

    WOS Identifier

    WOS:000251553700003

    ISSN

    0022-1198

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