Photolithographic patterning of C2C12 myotubes using vitronectin as growth substrate in serum-free medium

    Authors

    P. Molnar; W. S. Wang; A. Natarajan; J. W. Rumsey;J. J. Hickman

    Comments

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    Abbreviated Journal Title

    Biotechnol. Prog.

    Keywords

    SMOOTH-MUSCLE-CELLS; SKELETAL-MUSCLE; IN-VITRO; HIPPOCAMPAL-NEURONS; PRINTING METHOD; TISSUE; DIFFERENTIATION; MYOBLASTS; MOUSE; EXPRESSION; Biotechnology & Applied Microbiology; Food Science & Technology

    Abstract

    The C2C12 cell line is frequently used as a model of skeletal muscle differentiation. In our serum-free defined culture system, differentiation of C2C12 cells into myotubes required surface-bound signals such as substrate-adsorbed vitronectin or laminin. On the basis of this substrate requirement of myotube formation, we developed a photolithography-based method to pattern C2C12 myotubes, where myotubes formed exclusively on vitronectin surface patterns. We have determined that the optimal line width to form single myotubes is approximately 30 mu m. To illustrate a possible application of this method, we patterned myotubes on the top of commercial substrate-embedded microelectrodes. In contrast to previous experiments where cell patterning was achieved by selective attachment of the cells to patterned surfaces in a medium that contained all of the factors necessary for differentiation, this study illustrates that surface patterning of a signaling molecule, which is essential for skeletal muscle differentiation in a defined system, can result in the formation of aligned myotubes on the patterns. This technique is being developed for applications in cell biology, tissue engineering, and robotics.

    Journal Title

    Biotechnology Progress

    Volume

    23

    Issue/Number

    1

    Publication Date

    1-1-2007

    Document Type

    Article

    Language

    English

    First Page

    265

    Last Page

    268

    WOS Identifier

    WOS:000243927600036

    ISSN

    8756-7938

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