MCP-1 induces cardioprotection against ischaemia/reperfusion injury: role of reactive oxygen species

    Authors

    H. Morimoto; M. Hirose; M. Takahashi; M. Kawaguchi; H. Ise; P. E. Kolattukudy; M. Yamada;U. Ikeda

    Comments

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    Abbreviated Journal Title

    Cardiovasc. Res.

    Keywords

    apoptosis; cytokines; infection/inflammation; myocytes; oxygen radicals; MONOCYTE CHEMOATTRACTANT PROTEIN-1; EXPERIMENTAL MYOCARDIAL-INFARCTION; SMOOTH-MUSCLE CELLS; MIGRATION IN-VITRO; K-ATP CHANNELS; NADPH OXIDASE; CARDIAC OVEREXPRESSION; INFLAMMATORY RESPONSE; RADICAL GENERATION; REPERFUSION INJURY; Cardiac & Cardiovascular Systems

    Abstract

    Aims Monocyte chemoattractant protein-1 (MCP-1: CCL2) has been demonstrated to be involved in the pathophysiology of ischaemic heart disease; however, the precise rote of MCP-1 in ischaemia/reperfusion (I/R) injury is controversial. Here, we investigated the role of cardiac MCP-1 expression on left ventricular (I]V) dysfunction after global I/R in Langendorff-perfused hearts isolated from transgenic mice expressing the mouse JE-MCP-1 gene under the control of the alpha-cardiac myosin heavy chain promoter (MHC/MCP-1 mice). Methods and results In vitro experiments showed that MCP-1 prevented the apoptosis of murine neonatal cardiomyocytes after hypoxia/reoxygenation. I/R significantly increased the mRNA expression of MCP-1 in the Langendorff-perfused hearts of wild-type mice. Cardiac MCP-1 overexpression in the MHC/MCP-1 mice improved LV dysfunction after I/R without affecting coronary flow; in particular, it ameliorated LV diastolic pressure after reperfusion. This improvement was independent of both sarcolemmal. and mitochondrial K-ATP channels. Cardiac MCP-1 overexpression prevented superoxide generation in the I/R hearts, and these hearts showed decreased expression of the NADPH oxidase family proteins Nox1, gp91phox, and Nox3 compared with the hearts of wild-type mice. Further, superoxide dismutase activity in the hearts of MHC/MCP-1 mice was significantly increased compared with that in the hearts of wild-type mice. Conclusion These findings suggest that cardiac MCP-1 prevented LV dysfunction after global I/R through a reactive oxygen species-dependent but K-ATP channel-independent pathway; this provides new insight into the beneficial role of MCP-1 in the pathophysiology of ischaemic heart diseases.

    Journal Title

    Cardiovascular Research

    Volume

    78

    Issue/Number

    3

    Publication Date

    1-1-2008

    Document Type

    Article

    Language

    English

    First Page

    554

    Last Page

    562

    WOS Identifier

    WOS:000256738000019

    ISSN

    0008-6363

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