Abbreviated Journal Title
Infect. Immun.
Keywords
ENTEROAGGREGATIVE ESCHERICHIA-COLI; TRANS-GOLGI NETWORK; PHOSPHATIDYLINOSITOL 3-KINASE ACTIVITY; ENDOPLASMIC-RETICULUM; DIPHTHERIA-TOXIN; CHOLERA-TOXIN; BREFELDIN-A; INTRACELLULAR TRAFFICKING; AUTOTRANSPORTER PROTEINS; WORTMANNIN ALTERS; Immunology; Infectious Diseases
Abstract
The plasmid-encoded toxin (Pet) of enteroaggregative Escherichia coli is a 104-kDa autotransporter protein that exhibits proteolytic activity against the actin-binding protein alpha-fodrin. Intracellular cleavage of epithelial fodrin by Pet disrupts the actin cytoskeleton, causing both cytotoxic and enterotoxic effects. Intoxication requires the serine protease activity of Pet and toxin endocytosis from clathrin-coated pits. The additional events in the intracellular trafficking of Pet are largely uncharacterized. Here, we determined by confocal microscopy that internalized Pet is transferred from the early endosomes to the Golgi apparatus and then travels to the endoplasmic reticulum (ER). Pet associates with the Sec61p translocon before it moves into the cytosol as an intact, 104-kDa protein. This translocation process contrasts with the export of other ER-translocating toxins, in which only the catalytic A subunit of the AB toxin enters the cytosol. However, like intoxication with these AB toxins, Pet intoxication was inhibited in a subset of mutant CHO cell lines with aberrant activity in the ER-associated degradation pathway of ER-to-cytosol translocation. This is the first report which documents the cell surface-to-ER and ER-to-cytosol trafficking of a bacterial non-AB toxin.
Journal Title
Infection and Immunity
Volume
75
Issue/Number
5
Publication Date
1-1-2007
Document Type
Article
Language
English
First Page
2101
Last Page
2109
WOS Identifier
ISSN
0019-9567
Recommended Citation
Navarro-García, Fernando; Canizalez-Roman, Adrián; Burlingame, Kaitlin E.; Teter, Ken; and Vidal, Jorge E., "Pet, a non-AB toxin, is transported and translocated into epithelial cells by a retrograde trafficking pathway" (2007). Faculty Bibliography 2000s. 7465.
https://stars.library.ucf.edu/facultybib2000/7465
Comments
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