Title

Surface-derivatized nanoceria with human carbonic anhydrase II inhibitors and fluorophores: A potential drug delivery device

Authors

Authors

S. Patil; S. Reshetnikov; M. K. Haldar; S. Seal;S. Mallik

Comments

Authors: contact us about adding a copy of your work at STARS@ucf.edu

Abbreviated Journal Title

J. Phys. Chem. C

Keywords

BINDING GROUPS; QUANTUM DOTS; NANOPARTICLES; FLUORESCENT; PROBES; MICROSPHERES; SULFONAMIDES; DISPOSITION; CONSTANTS; EFFICIENT; Chemistry, Physical; Nanoscience & Nanotechnology; Materials Science, ; Multidisciplinary

Abstract

Human carbonic anhydrase (hCAII) is a metalloenzyme that catalyzes the reversible hydration of carbon dioxide to bicarbonate and is associated with glaucoma (a major cause of blindness). The present study focuses on the use of cerium oxide nanoparticles (nanoceria) as a potential delivery device for hCAII inhibitors. Carboxybenzenesulfonamide, an inhibitor of the hCAII enzyme, was attached to nanoceria particles using epichlorohydrin as an intermediate linkage. Along with the CA inhibitor, a fluorophore (carboxyfluorescein) was also attached on the nanoparticles to enable the tracking of the nanoparticles in vitro as well as in vivo. X-ray photoelectron spectroscopic studies carried out at each reaction step confirmed the successful derivatization of the nanoceria particles. The attachment of carboxyfluorescein was also confirmed by confocal fluorescence microscopy. Preliminary studies suggest that carboxybenzenesulfonamide-functionalized nanoceria retains its inhibitory potency for hCAII.

Journal Title

Journal of Physical Chemistry C

Volume

111

Issue/Number

24

Publication Date

1-1-2007

Document Type

Article

Language

English

First Page

8437

Last Page

8442

WOS Identifier

WOS:000247215200008

ISSN

1932-7447

Share

COinS