Surface-derivatized nanoceria with human carbonic anhydrase II inhibitors and fluorophores: A potential drug delivery device

    Authors

    S. Patil; S. Reshetnikov; M. K. Haldar; S. Seal;S. Mallik

    Comments

    Authors: contact us about adding a copy of your work at STARS@ucf.edu

    Abbreviated Journal Title

    J. Phys. Chem. C

    Keywords

    BINDING GROUPS; QUANTUM DOTS; NANOPARTICLES; FLUORESCENT; PROBES; MICROSPHERES; SULFONAMIDES; DISPOSITION; CONSTANTS; EFFICIENT; Chemistry, Physical; Nanoscience & Nanotechnology; Materials Science, ; Multidisciplinary

    Abstract

    Human carbonic anhydrase (hCAII) is a metalloenzyme that catalyzes the reversible hydration of carbon dioxide to bicarbonate and is associated with glaucoma (a major cause of blindness). The present study focuses on the use of cerium oxide nanoparticles (nanoceria) as a potential delivery device for hCAII inhibitors. Carboxybenzenesulfonamide, an inhibitor of the hCAII enzyme, was attached to nanoceria particles using epichlorohydrin as an intermediate linkage. Along with the CA inhibitor, a fluorophore (carboxyfluorescein) was also attached on the nanoparticles to enable the tracking of the nanoparticles in vitro as well as in vivo. X-ray photoelectron spectroscopic studies carried out at each reaction step confirmed the successful derivatization of the nanoceria particles. The attachment of carboxyfluorescein was also confirmed by confocal fluorescence microscopy. Preliminary studies suggest that carboxybenzenesulfonamide-functionalized nanoceria retains its inhibitory potency for hCAII.

    Journal Title

    Journal of Physical Chemistry C

    Volume

    111

    Issue/Number

    24

    Publication Date

    1-1-2007

    Document Type

    Article

    Language

    English

    First Page

    8437

    Last Page

    8442

    WOS Identifier

    WOS:000247215200008

    ISSN

    1932-7447

    Share

    COinS