Title

Practical issues in stem cell therapy for Alzheimer's disease

Authors

Authors

K. Sugaya; Y. D. Kwak; O. Ohmitsu; A. Marutle; N. H. Greig;E. Choumrina

Comments

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Abbreviated Journal Title

Curr. Alzheimer Res.

Keywords

stem cell; beta-amyloid; amyloid precursor protein; neural; differentiation; neurogenesis; gliosis; (+)-phenserine; AMYLOID PRECURSOR PROTEIN; ADULT SUBVENTRICULAR ZONE; FIBRILLARY ACIDIC; PROTEIN; EMBRYONAL CARCINOMA-CELLS; BINDING PROTEIN; NERVOUS-SYSTEM; HUMAN NEURONS; MOUSE-BRAIN; IN-VITRO; DIFFERENTIATION; Clinical Neurology; Neurosciences

Abstract

We have demonstrated that aged animals show significant improvements in cognitive function and neurogenesis after brain transplantation of human neural stem cells or of human adult mesenchymal stem cells that have been dedifferentiated by transfection of the embryonic stem cell gene. We have also demonstrated that peripheral administration of a pyrimidine derivative increased cognition, endogenous brain stem cell proliferation and neurogenesis. These results indicate a bright future for stem cell therapies in Alzheimer's disease (AD). Before this is realized, however, we need to consider the affect of AD pathology on stem cell biology to establish an effective stem cell therapy for this disease. Although amyloid-beta (A beta) deposition is a hallmark of AD, an absence of a phenotype in the beta-amyloid precursor protein (APP) knockout mouse, might lead one to underestimate the potential physiological functions of APP and suggest that it is unessential or can be compensated for. We have found, however, that APP is needed for differentiation of neural stem cells (NSCs) in vitro, and that NSCs transplanted into a APP-knockout mouse did not migrate or differentiate - indicating that APP plays an important role in differentiation or migration process of NSCs in the brain. Then again, treatment with high a concentration of APP or its over-expression increased glial differentiation of NSCs. Human NSCs transplanted into APP-transgenic mouse brain exhibited less neurogenesis and active gliosis around the plaque like fort-nations. Treatment of such animals with the compound, (+)-phenserine, that is known to reduce APP protein levels, increased neurogenesis and suppressed gliosis. These results suggest APP levels can regulate NSC biology in the adult brain, that altered APP metabolism in Down syndrome or AD may have implications for the pathophysiology of these diseases, and that a combination of stem cell therapy and regulation of APP levels could provide a treatment strategy for these disorders.

Journal Title

Current Alzheimer Research

Volume

4

Issue/Number

4

Publication Date

1-1-2007

Document Type

Article

Language

English

First Page

370

Last Page

377

WOS Identifier

WOS:000250494700006

ISSN

1567-2050

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