Chalcogenide waveguide structures as substrates and guiding layers for evanescent wave Raman spectroscopy of bacteriorhodopsin

    Authors

    A. Pope; A. Schulte; Y. Guo; L. K. Ono; B. R. Cuenya; C. Lopez; K. Richardson; K. Kitanovski;T. Winningham

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    Abbreviated Journal Title

    Vib. Spectrosc.

    Keywords

    Raman spectroscopy; chalcogenide glass waveguides; bacteriorhodopsin; FILMS; PROTEIN; Chemistry, Analytical; Chemistry, Physical; Spectroscopy

    Abstract

    We investigate composite structures where a chalcogenide waveguide provides the substrate and the guiding layer for a bacteriorhodopsin film whose Raman spectrum is desired. Plasma exposure of the chalcogenide substrate allows for a hydrophilic surface to which organic layers can easily adhere. Surface morphology, structure, and chemical composition are characterized by X-ray photoelectron spectroscopy. A 785 nm beam from a Ti:Sapphire laser was coupled into the As2S3 waveguide and the evanescent field was employed to excite Raman scattering in the bacteriorhodopsin film. The Raman spectrum of the bacteriorhodopsin film in the fingerprint region is indicative of the native conformation and it shows that the protein is in the light adapted state. (C) 2006 Elsevier B.V. All rights reserved.

    Journal Title

    Vibrational Spectroscopy

    Volume

    42

    Issue/Number

    2

    Publication Date

    1-1-2006

    Document Type

    Article; Proceedings Paper

    Language

    English

    First Page

    249

    Last Page

    253

    WOS Identifier

    WOS:000242764300014

    ISSN

    0924-2031

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