Title

Hammerhead ribozymes: Biochemical and chemical considerations

Authors

Authors

J. Goodchild

Comments

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Abbreviated Journal Title

Curr. Opin. Mol. Ther.

Keywords

chemical modification; facilitator oligonucleotide; hammerhead; mRNA; knockout; nuclease resistance; ribozyme; VASCULAR SMOOTH-MUSCLE; LEUKOCYTE-TYPE 12-LIPOXYGENASE; NUCLEASE-RESISTANT RIBOZYMES; HUMAN-IMMUNODEFICIENCY-VIRUS; RESONANCE; ENERGY-TRANSFER; SELF-CLEAVAGE REACTION; ANTI-RAS RIBOZYME; MESSENGER-RNA; IN-VITRO; OLIGONUCLEOTIDE FACILITATORS; Biotechnology & Applied Microbiology; Medicine, Research & Experimental

Abstract

Ribozymes can be used to cleave specific mRNAs so as to prevent their translation. This presents An alternative to the use of antisense oligonucleotides for drag target validation or therapeutic purposes. The present review covers only one class of ribozymes, the hammerheads, which are small enough for chemical synthesis. Following an account of their structure and ability to catalyze the cleavage of target RNA is a discussion of some problems associated with their use as exogenous therapeutics. Chemical modifications that are used to address these issues are described.

Journal Title

Current Opinion in Molecular Therapeutics

Volume

2

Issue/Number

3

Publication Date

1-1-2000

Document Type

Review

Language

English

First Page

272

Last Page

281

WOS Identifier

WOS:000088671900006

ISSN

1464-8431

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