Hammerhead ribozymes: Biochemical and chemical considerations

    Authors

    J. Goodchild

    Comments

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    Abbreviated Journal Title

    Curr. Opin. Mol. Ther.

    Keywords

    chemical modification; facilitator oligonucleotide; hammerhead; mRNA; knockout; nuclease resistance; ribozyme; VASCULAR SMOOTH-MUSCLE; LEUKOCYTE-TYPE 12-LIPOXYGENASE; NUCLEASE-RESISTANT RIBOZYMES; HUMAN-IMMUNODEFICIENCY-VIRUS; RESONANCE; ENERGY-TRANSFER; SELF-CLEAVAGE REACTION; ANTI-RAS RIBOZYME; MESSENGER-RNA; IN-VITRO; OLIGONUCLEOTIDE FACILITATORS; Biotechnology & Applied Microbiology; Medicine, Research & Experimental

    Abstract

    Ribozymes can be used to cleave specific mRNAs so as to prevent their translation. This presents An alternative to the use of antisense oligonucleotides for drag target validation or therapeutic purposes. The present review covers only one class of ribozymes, the hammerheads, which are small enough for chemical synthesis. Following an account of their structure and ability to catalyze the cleavage of target RNA is a discussion of some problems associated with their use as exogenous therapeutics. Chemical modifications that are used to address these issues are described.

    Journal Title

    Current Opinion in Molecular Therapeutics

    Volume

    2

    Issue/Number

    3

    Publication Date

    1-1-2000

    Document Type

    Review

    Language

    English

    First Page

    272

    Last Page

    281

    WOS Identifier

    WOS:000088671900006

    ISSN

    1464-8431

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