Title

Down-regulation of prostasin serine protease: A potential invasion suppressor in prostate cancer

Authors

Authors

L. M. Chen; G. B. Hodge; L. A. Guarda; J. L. Welch; N. M. Greenberg;K. X. Chai

Comments

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Abbreviated Journal Title

Prostate

Keywords

cell line; gene expression; immunohistochemistry; prostatectomy; transfection; MOLECULAR-CLONING; LOCALIZATION; PROTEINASE; EXPRESSION; MOUSE; CELLS; Endocrinology & Metabolism; Urology & Nephrology

Abstract

BACKGROUND. Prostasin is a serine protease predominantly expressed in normal prostate epithelial cells. The biological function of prostasin has not been determined. METHODS. Western blot and RT-PCR analyses were used to examine the expression of prostasin in prostate cancer cell lines. Immunohistochemistry was used to evaluate prostasin protein expression in human prostate cancer. An in vitro Matrigel invasion assay was used to test the invasiveness Of prostate cancer cell lines forced to express recombinant prostasin. RESULTS. Both prostasin protein and mRNA were found to be expressed in normal human prostate epithelial cells and a non-invasive human prostate cancer cell line, the LNCaP, but neither was found in invasive human prostate cancer cell lines DU-145 and PC-3. Prostasin mRNA expression was absent in invasive prostate cancer cell lines of a transgenic mouse model. Immunohistochemistry analysis showed that prostasin protein expression is downregulated in high-grade prostate cancer. Transfection of DU-145 and PC-3 cells with a full-length human prostasin cDNA restored prostasin expression and reduced the in vitro invasiveness by 68 and 42%, respectively. CONCLUSIONS. Our data indicate that prostasin may be implicated in normal prostate biology and is able to suppress prostate cancer invasion in vitro. (C) 2001 Wiley-Liss, Inc.

Journal Title

Prostate

Volume

48

Issue/Number

2

Publication Date

1-1-2001

Document Type

Article

Language

English

First Page

93

Last Page

103

WOS Identifier

WOS:000169716000004

ISSN

0270-4137

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