Title
Distribution of DNA in cationic liposome complexes probed by Raman microscopy
Abbreviated Journal Title
Langmuir
Keywords
ATOMIC-FORCE MICROSCOPY; GENE-TRANSFER; CONDENSATION; PHASE; EXPRESSION; TRANSITION; DYNAMICS; DELIVERY; THERAPY; HUMANS; Chemistry, Multidisciplinary; Chemistry, Physical; Materials Science, ; Multidisciplinary
Abstract
The potential application of Raman microscopy to image the distribution of DNA inside lipid-DNA complexes was examined. To test this, we mixed DNA with DOTMA/DOPE liposome vesicles and imaged the distribution of DNA in the liposome complex by using the characteristic DNA guanine peak as a marker. The Raman intensity map indicates that the concentration of DNA molecules is higher near the core of the DOTMA/DOPE liposome vesicle. This result demonstrates that this technique is a viable technique to monitor DNA distribution inside liposomes.
Journal Title
Langmuir
Volume
17
Issue/Number
3
Publication Date
1-1-2001
Document Type
Letter
DOI Link
Language
English
First Page
571
Last Page
573
WOS Identifier
ISSN
0743-7463
Recommended Citation
"Distribution of DNA in cationic liposome complexes probed by Raman microscopy" (2001). Faculty Bibliography 2000s. 8122.
https://stars.library.ucf.edu/facultybib2000/8122
Comments
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