Distribution of DNA in cationic liposome complexes probed by Raman microscopy

    Authors

    H. Matsui;S. Pan

    Comments

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    Abbreviated Journal Title

    Langmuir

    Keywords

    ATOMIC-FORCE MICROSCOPY; GENE-TRANSFER; CONDENSATION; PHASE; EXPRESSION; TRANSITION; DYNAMICS; DELIVERY; THERAPY; HUMANS; Chemistry, Multidisciplinary; Chemistry, Physical; Materials Science, ; Multidisciplinary

    Abstract

    The potential application of Raman microscopy to image the distribution of DNA inside lipid-DNA complexes was examined. To test this, we mixed DNA with DOTMA/DOPE liposome vesicles and imaged the distribution of DNA in the liposome complex by using the characteristic DNA guanine peak as a marker. The Raman intensity map indicates that the concentration of DNA molecules is higher near the core of the DOTMA/DOPE liposome vesicle. This result demonstrates that this technique is a viable technique to monitor DNA distribution inside liposomes.

    Journal Title

    Langmuir

    Volume

    17

    Issue/Number

    3

    Publication Date

    1-1-2001

    Document Type

    Letter

    Language

    English

    First Page

    571

    Last Page

    573

    WOS Identifier

    WOS:000166765400002

    ISSN

    0743-7463

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