Title

Isolation and characterization of tetranucleotide microsatellites from the leatherback turtle (Dermochelys coriacea)

Authors

Authors

T. I. Alstad; B. M. Shamblin; D. A. Bagley; L. M. Ehrhart;C. J. Nairn

Comments

Authors: contact us about adding a copy of your work at STARS@ucf.edu

Abbreviated Journal Title

Conserv. Genet. Resour.

Keywords

Leatherback turtle; Dermochelys coriacea; Microsatellite; Population; structure; Individual identity; CARETTA-CARETTA; AMPLIFICATION; MARKERS; Biodiversity Conservation; Genetics & Heredity

Abstract

The leatherback turtle (Dermochelys coriacea) is a globally endangered marine species. Numerous questions regarding life history and demographics that are of conservation interest remain and many of these can be addressed through the use of highly polymorphic nuclear markers. We describe primers and polymerase chain reaction conditions to amplify 19 tetranucleotide microsatellite loci from the leatherback turtle. The primers were tested on samples from 22 females that nested at Archie Carr National Wildlife Refuge, Melbourne Beach, Florida, USA. The primers developed in this study yielded an average of 9.4 alleles per locus (range of 5-19) and an average observed heterozygosity of 0.84 (range 0.36-1.00). These markers should prove useful in supplementing existing markers for individual and population level analyses.

Journal Title

Conservation Genetics Resources

Volume

3

Issue/Number

3

Publication Date

1-1-2011

Document Type

Article

Language

English

First Page

457

Last Page

460

WOS Identifier

WOS:000291169700014

ISSN

1877-7252

Share

COinS