Title

Rayleigh and Brillouin scattering in a lysozyme-water mixture: An unusual behavior around 343 K

Authors

Authors

A. Asenbaum; C. Pruner; H. W. Schroer; K. Platzer; L. Bieler; H. Brandstetter; W. Schirmacher; A. Schulte;E. Wilhelm

Comments

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Abbreviated Journal Title

J. Mol. Liq.

Keywords

Rayleigh scattering; Brillouin scattering; Lysozyme-water mixtures; EGG-WHITE LYSOZYME; LIQUID-PHASE-SEPARATION; FABRY-PEROT-INTERFEROMETER; MOLTEN GLOBULE STATE; X-RAY-SCATTERING; LIGHT-SCATTERING; BIOLOGICAL; MACROMOLECULES; THERMAL-DENATURATION; SCANNING CALORIMETRY; SUPERCOOLED; WATER; Chemistry, Physical; Physics, Atomic, Molecular & Chemical

Abstract

This article describes Rayleigh and Brillouin light scattering studies on a lysozyme-water mixture from 293 K to 355 K. The scattering intensities from this system are compared with those from a sodium acetate buffer used to dissolve the lysozyme. It is found that in the vicinity of 343 K the lysozyme-water mixture becomes opalescent, and the intensity of the Brillouin peaks decreases and almost vanishes, to be restored at temperatures above 343 K. Around the same temperature the intensity of the central, unshifted Rayleigh peak, however, increases strongly. No such behavior was observed for the sodium acetate buffer. The analysis of the experimental data indicates an irreversible transition, near 343 K. from a metastable buffered system containing folded lysozyme molecules to a multimolecular gel of thermally unfolded lysozyme molecules. (C) 2010 Elsevier B.V. All rights reserved.

Journal Title

Journal of Molecular Liquids

Volume

158

Issue/Number

1

Publication Date

1-1-2011

Document Type

Article

Language

English

First Page

7

Last Page

12

WOS Identifier

WOS:000286788100002

ISSN

0167-7322

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