mRNA profiling for the identification of blood-Results of a collaborative EDNAP exercise

    Authors

    C. Haas; E. Hanson; W. Bar; R. Banemann; A. M. Bento; A. Berti; E. Borges; C. Bouakaze; A. Carracedo; M. Carvalho; A. Choma; M. Dotsch; M. Duriancikova; P. Hoff-Olsen; C. Hohoff; P. Johansen; P. A. Lindenbergh; B. Loddenkotter; B. Ludes; O. Maronas; N. Morling; H. Niederstatter; W. Parson; G. Patel; C. Popielarz; E. Salata; P. M. Schneider; T. Sijen; B. Sviezena; L. Zatkalikova;J. Ballantyne

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    Abbreviated Journal Title

    Forensic Sci. Int.-Genet.

    Keywords

    Forensic science; mRNA profiling; Body fluid identification; Blood; EDNAP exercise; BODY-FLUID IDENTIFICATION; RT-PCR; MENSTRUAL BLOOD; SALIVA STAINS; MARKERS; SEMEN; Genetics & Heredity; Medicine, Legal

    Abstract

    A collaborative exercise on mRNA profiling for the identification of blood was organized by the European DNA Profiling Group (EDNAP). Seven blood samples and one blood dilution series were analyzed by the participating laboratories for the reportedly blood-specific markers HBB, SPTB and PBGD, using different kits, chemistries and instrumentation. The results demonstrate that HBB is expressed abundantly in blood, SPTB moderately and PBGD significantly less. All but one of the 16 participating laboratories were able to successfully isolate and detect RNA from the dried bloodstains even though a majority of the laboratories had no prior experience with RNA. Despite some expected variation in sensitivity between laboratories, the method proved to be reproducible and sensitive using different analysis strategies. The results of this collaborative exercise support the potential use of mRNA profiling as an alternative to conventional serological tests. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

    Journal Title

    Forensic Science International-Genetics

    Volume

    5

    Issue/Number

    1

    Publication Date

    1-1-2011

    Document Type

    Article

    Language

    English

    First Page

    21

    Last Page

    26

    WOS Identifier

    WOS:000286134200018

    ISSN

    1872-4973

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