Title

Incorporation of the HIV-1 Microbicide Cyanovirin-N in a Food Product

Authors

Authors

M. Li; D. L. Patton; Y. Cosgrove-Sweeney; D. Ratner; L. C. Rohan; A. M. Cole; P. M. Tarwater; P. Gupta;B. Ramratnam

Comments

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Abbreviated Journal Title

Jaids

Keywords

cyanovirin-N; CV-N; HIV-1; lactic acid bacteria; microbicide; pigtail; macaque; VIRUS-INACTIVATING PROTEIN; VAGINAL SHIV CHALLENGE; LACTOBACILLUS-JENSENII; TRANSMISSION; MACAQUES; GP120; PROTECTION; INHIBITORS; BACTERIA; SECRETE; Immunology; Infectious Diseases

Abstract

An urgent need exists for HIV-1 microbicides. Here, we describe the in vivo testing of lactic acid bacteria bioengineered to secrete cyanovirin-N. We fed pigtail macaques a yogurt formulation that used bioengineered strains as a starter culture. Cyanovirin-N expression could be detected in the rectal vault during and immediately after feeding. Ex vivo viral challenge of rectal tissue biopsies revealed that peak viral burden was significantly lower in tissue obtained from experimental animals compared with control animals. Formulation of candidate compounds in lactic acid bacteria and their oral administration seems to be a feasible strategy for mucosal delivery of microbicides.

Journal Title

Jaids-Journal of Acquired Immune Deficiency Syndromes

Volume

58

Issue/Number

4

Publication Date

1-1-2011

Document Type

Article

Language

English

First Page

379

Last Page

384

WOS Identifier

WOS:000296920900014

ISSN

1525-4135

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