Incorporation of the HIV-1 Microbicide Cyanovirin-N in a Food Product

    Authors

    M. Li; D. L. Patton; Y. Cosgrove-Sweeney; D. Ratner; L. C. Rohan; A. M. Cole; P. M. Tarwater; P. Gupta;B. Ramratnam

    Comments

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    Abbreviated Journal Title

    Jaids

    Keywords

    cyanovirin-N; CV-N; HIV-1; lactic acid bacteria; microbicide; pigtail; macaque; VIRUS-INACTIVATING PROTEIN; VAGINAL SHIV CHALLENGE; LACTOBACILLUS-JENSENII; TRANSMISSION; MACAQUES; GP120; PROTECTION; INHIBITORS; BACTERIA; SECRETE; Immunology; Infectious Diseases

    Abstract

    An urgent need exists for HIV-1 microbicides. Here, we describe the in vivo testing of lactic acid bacteria bioengineered to secrete cyanovirin-N. We fed pigtail macaques a yogurt formulation that used bioengineered strains as a starter culture. Cyanovirin-N expression could be detected in the rectal vault during and immediately after feeding. Ex vivo viral challenge of rectal tissue biopsies revealed that peak viral burden was significantly lower in tissue obtained from experimental animals compared with control animals. Formulation of candidate compounds in lactic acid bacteria and their oral administration seems to be a feasible strategy for mucosal delivery of microbicides.

    Journal Title

    Jaids-Journal of Acquired Immune Deficiency Syndromes

    Volume

    58

    Issue/Number

    4

    Publication Date

    1-1-2011

    Document Type

    Article

    Language

    English

    First Page

    379

    Last Page

    384

    WOS Identifier

    WOS:000296920900014

    ISSN

    1525-4135

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