Detecting protein adsorption and binding using magnetic nanoparticle probes

    Authors

    L. Y. Ma; C. M. Wang;M. H. Zhang

    Comments

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    Abbreviated Journal Title

    Sens. Actuator B-Chem.

    Keywords

    Microcantilever; Magnetic nanoparticles; Protein; Biomarkers; Detection; BIOLOGICAL DETECTION; SENSORS; DNA; TECHNOLOGY; BIOSENSOR; ARRAY; Chemistry, Analytical; Electrochemistry; Instruments & Instrumentation

    Abstract

    Magnetic force induced microcantilever bending is used to enhance sensitivity of protein biomarker detection using magnetic nanoparticles. After modifying magnetic nanoparticles and microcantilever with antibodies, the existence of antigen in solution leads to immobilization of nanoparticles on microcantilever by forming antigen-antibody complexes. The microcantilever bends in the presence of magnetic field, and the magnitude of bending is dependent on strength of magnetic field and amount of immobilized magnetic nanoparticles. This method has been used to monitor protein adsorption, and detect protein biomarker in complex biological sample system, such as cell lysate. The magnetic induced bending offers high sensitivity, does not require an asymmetric surface modification on microcantilever, and can be used for the reliable detection of protein biomarkers in body biofluids. (C) 2011 Elsevier B.V. All rights reserved.

    Journal Title

    Sensors and Actuators B-Chemical

    Volume

    160

    Issue/Number

    1

    Publication Date

    1-1-2011

    Document Type

    Article

    Language

    English

    First Page

    650

    Last Page

    655

    WOS Identifier

    WOS:000298768100092

    ISSN

    0925-4005

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