Title

Epigenetic regulation of microRNA-375 and its role in melanoma development in humans

Authors

Authors

J. Mazar; D. DeBlasio; S. S. Govindarajan; S. J. Zhang;R. J. Perera

Comments

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Abbreviated Journal Title

FEBS Lett.

Keywords

Epigenetic regulation; MicroRNA; miR-375; Cell invasion; Human melanoma; CELL LINES; TRANSCRIPTION FACTOR; MALIGNANT-MELANOMA; TUMOR SUPPRESSORS; BREAST-CANCER; HOST GENE; EXPRESSION; DIFFERENTIATION; PROGRESSION; ONCOGENES; Biochemistry & Molecular Biology; Biophysics; Cell Biology

Abstract

To identify epigenetically regulated miRNAs in melanoma, we treated a stage 3 melanoma cell line WM1552C, with 5AzadC and/or 4-PBA. Several hypermethylated miRNAs were detected, one of which, miR-375, was highly methylated and was studied further. Minimal CpG island methylation was observed in melanocytes, keratinocytes, normal skin, and nevus but hypermethylation was observed in patient tissue samples from primary, regional, distant, and nodular metastatic melanoma. Ectopic expression of miR-375 inhibited melanoma cell proliferation, invasion, and cell motility, and induced cell shape changes, strongly suggesting that miR-375 may have an important function in the development and progression of human melanomas. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

Journal Title

Febs Letters

Volume

585

Issue/Number

15

Publication Date

1-1-2011

Document Type

Article

Language

English

First Page

2467

Last Page

2476

WOS Identifier

WOS:000293276000012

ISSN

0014-5793

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