Epigenetic regulation of microRNA-375 and its role in melanoma development in humans

    Authors

    J. Mazar; D. DeBlasio; S. S. Govindarajan; S. J. Zhang;R. J. Perera

    Comments

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    Abbreviated Journal Title

    FEBS Lett.

    Keywords

    Epigenetic regulation; MicroRNA; miR-375; Cell invasion; Human melanoma; CELL LINES; TRANSCRIPTION FACTOR; MALIGNANT-MELANOMA; TUMOR SUPPRESSORS; BREAST-CANCER; HOST GENE; EXPRESSION; DIFFERENTIATION; PROGRESSION; ONCOGENES; Biochemistry & Molecular Biology; Biophysics; Cell Biology

    Abstract

    To identify epigenetically regulated miRNAs in melanoma, we treated a stage 3 melanoma cell line WM1552C, with 5AzadC and/or 4-PBA. Several hypermethylated miRNAs were detected, one of which, miR-375, was highly methylated and was studied further. Minimal CpG island methylation was observed in melanocytes, keratinocytes, normal skin, and nevus but hypermethylation was observed in patient tissue samples from primary, regional, distant, and nodular metastatic melanoma. Ectopic expression of miR-375 inhibited melanoma cell proliferation, invasion, and cell motility, and induced cell shape changes, strongly suggesting that miR-375 may have an important function in the development and progression of human melanomas. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

    Journal Title

    Febs Letters

    Volume

    585

    Issue/Number

    15

    Publication Date

    1-1-2011

    Document Type

    Article

    Language

    English

    First Page

    2467

    Last Page

    2476

    WOS Identifier

    WOS:000293276000012

    ISSN

    0014-5793

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