Sustained protection against photoreceptor degeneration in tubby mice by intravitreal injection of nanoceria

    Authors

    X. Cai; S. A. Sezate; S. Seal;J. F. McGinnis

    Comments

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    Abbreviated Journal Title

    Biomaterials

    Keywords

    Tubby mouse; Nanoceria; Antioxidant; Gene expression; Retinal; degeneration; Longevity; ENDOPLASMIC-RETICULUM STRESS; CERIUM OXIDE NANOPARTICLES; OXIDATIVE; STRESS; RETINAL DEGENERATION; CELL-DEATH; DRUG-DELIVERY; OPSIN; MISLOCALIZATION; MACULAR DEGENERATION; RETINITIS-PIGMENTOSA; REDOX STATE; Engineering, Biomedical; Materials Science, Biomaterials

    Abstract

    We previously reported that nanoceria can slow retinal degeneration in the tubby mouse for two weeks by multiple systemic injections. However, the long-term protection of retinal structure and function by directly deliver of nanoceria to the eye had not been explored. In this study, 172 ng of nanoceria in 1 mu l saline (1 mM) were intravitreally injected into tubby P7 pups and assays were performed at P28, P49, P80 and P120. The expression of antioxidant associated genes and photoreceptor-specific genes was significantly up regulated, the mislocalization of rod and cone opsins was decreased, and retinal structure and function were protected. These findings demonstrate that nanoceria can function as catalytic antioxidants in vivo and may be broad spectrum therapeutic agents for multiple types of ocular diseases. (C) 2012 Elsevier Ltd. All rights reserved.

    Journal Title

    Biomaterials

    Volume

    33

    Issue/Number

    34

    Publication Date

    1-1-2012

    Document Type

    Article

    Language

    English

    First Page

    8771

    Last Page

    8781

    WOS Identifier

    WOS:000310390100003

    ISSN

    0142-9612

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