Authors

M. Hossain; C. M. Wang;M. Su

Comments

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“This article may be downloaded for personal use only. Any other use requires prior permission of the author and AIP Publishing. This article appeared in this linked citation and may be found at Applied Physics Letters."

Abbreviated Journal Title

Appl. Phys. Lett.

Keywords

PROSTATE-CANCER; BREAST; TISSUES; ELEMENTS; COLON; BONE; LEAD; Physics, Applied

Abstract

Multiple DNA and protein biomarkers have been detected based on characteristic x-ray fluorescence of a panel of metal and alloy nanoparticles, which are modified with ligands of biomarkers to create a one-to-one correspondence and immobilized on ligand-modified substrates after forming complexes with target biomarkers in three-strand or sandwich configuration. By determining the presence and concentration of nanoparticles using x-ray fluorescence, the nature and amount of biomarkers can be detected with limits of 1 nM for DNA and 1 ng/ml for protein. By combining high penetrating ability of x-rays, this method allows quantitative imaging of multiple biomarkers.

Journal Title

Applied Physics Letters

Volume

97

Issue/Number

26

Publication Date

1-1-2010

Document Type

Article

Language

English

First Page

3

WOS Identifier

WOS:000285768100093

ISSN

0003-6951

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