Performance Evaluation and Optimization of Multiplex PCRs for the Highly Discriminating OSU 10-Locus Set Y-STRs

    Authors

    E. Hanson; J. L. Maybruck; J. Ballantyne;P. A. Fuerst

    Comments

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    Abbreviated Journal Title

    J. Forensic Sci.

    Keywords

    forensic science; short tandem repeats; microsatellites; Y-chromosome; DYS448; DYS487; DYS488; DYS504; DYS576; DYS657; DYS685; DYS688; DYS703; DYS707; FORENSIC CASEWORK; POPULATION GROUPS; UNITED-STATES; CHROMOSOME; SYSTEM; Medicine, Legal

    Abstract

    In a previous study, a new set of Y-chromosome short tandem repeats, the OSU 10-locus set (MPM1 and MPM2), was shown to have a higher discrimination power when evaluated against the 10 SWGDAM loci on a common population panel. Here, we describe the optimization of the multiplex reactions using dye-labeled primers followed by performance evaluations. The loci exhibited high precision, human male specificity, reliability in different body fluids, high sensitivity, stability, and the ability to amplify nonprobative casework and mixture samples. Stutter for the all of the loci, with the exception of the highly polymorphic locus DYS688, was similar to that observed for autosomal loci. The results of the performance evaluations reinforced the utility of these loci.

    Journal Title

    Journal of Forensic Sciences

    Volume

    57

    Issue/Number

    1

    Publication Date

    1-1-2012

    Document Type

    Article

    Language

    English

    First Page

    52

    Last Page

    59

    WOS Identifier

    WOS:000298793800009

    ISSN

    0022-1198

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