Title
Performance Evaluation and Optimization of Multiplex PCRs for the Highly Discriminating OSU 10-Locus Set Y-STRs
Abbreviated Journal Title
J. Forensic Sci.
Keywords
forensic science; short tandem repeats; microsatellites; Y-chromosome; DYS448; DYS487; DYS488; DYS504; DYS576; DYS657; DYS685; DYS688; DYS703; DYS707; FORENSIC CASEWORK; POPULATION GROUPS; UNITED-STATES; CHROMOSOME; SYSTEM; Medicine, Legal
Abstract
In a previous study, a new set of Y-chromosome short tandem repeats, the OSU 10-locus set (MPM1 and MPM2), was shown to have a higher discrimination power when evaluated against the 10 SWGDAM loci on a common population panel. Here, we describe the optimization of the multiplex reactions using dye-labeled primers followed by performance evaluations. The loci exhibited high precision, human male specificity, reliability in different body fluids, high sensitivity, stability, and the ability to amplify nonprobative casework and mixture samples. Stutter for the all of the loci, with the exception of the highly polymorphic locus DYS688, was similar to that observed for autosomal loci. The results of the performance evaluations reinforced the utility of these loci.
Journal Title
Journal of Forensic Sciences
Volume
57
Issue/Number
1
Publication Date
1-1-2012
Document Type
Article
Language
English
First Page
52
Last Page
59
WOS Identifier
ISSN
0022-1198
Recommended Citation
"Performance Evaluation and Optimization of Multiplex PCRs for the Highly Discriminating OSU 10-Locus Set Y-STRs" (2012). Faculty Bibliography 2010s. 2714.
https://stars.library.ucf.edu/facultybib2010/2714
Comments
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