Bone morphogenetic protein 7 polarizes THP-1 cells into M2 macrophages

    Authors

    C. Rocher; R. Singla; P. K. Singal; S. Parthasarathy;D. K. Singla

    Comments

    Authors: contact us about adding a copy of your work at STARS@ucf.edu

    Abbreviated Journal Title

    Can. J. Physiol. Pharmacol.

    Keywords

    atherosclerosis; macrophages; monocytes; IL-10; ATHEROSCLEROSIS; MONOCYTES; GROWTH; BMP-7; Pharmacology & Pharmacy; Physiology

    Abstract

    It was hypothesized that monocyte treatment with bone morphogenetic protein 7 (BMP7) would significantly enhance monocyte polarization into M2 macrophages as well as increasing the levels of anti-inflammatory cytokines. In a cell culture system using monocytes (human acute monocytic leukemia cell line THP-1), we studied the effects of BMP7 on monocytes polarizing into M2 macrophages. The data demonstrate that THP-1 cells contain a BMP type II receptor (BMPR2), and that its activation is significantly (p < 0.05) increased following treatment with BMP7. Furthermore, there was an increase of M2 macrophages, BMPR2, and anti-inflammatory cytokines interleukin (IL)-10 and IL-1ra compared with the respective controls. Moreover, treatment with BMP7 caused a significant (p < 0.05) decrease in the levels of pro-inflammatory cytokines IL-6, tumour necrosis factor (TNF-alpha), and monocyte chemotactic protein-1 (MCP-1), compared with the controls. In conclusion, we suggest for the first time that BMP7 has a unique potential to polarize monocytes into M2 macrophages, required for tissue repair, which will have significant applications for the treatment of atherosclerosis.

    Journal Title

    Canadian Journal of Physiology and Pharmacology

    Volume

    90

    Issue/Number

    7

    Publication Date

    1-1-2012

    Document Type

    Article

    Language

    English

    First Page

    947

    Last Page

    951

    WOS Identifier

    WOS:000306110100014

    ISSN

    0008-4212

    Share

    COinS