Authors

T. E. Rogers; S. F. Ataide; K. Dare; A. Katz; S. Seveau; H. Roy;M. Ibba

Comments

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Abbreviated Journal Title

PLoS One

Keywords

REGULATED SMALL RNA; NITRIC-OXIDE; FUNCTIONAL IMPLICATIONS; ESCHERICHIA-COLI; IRON-METABOLISM; BACTERIA; SUBTILIS; GENES; MECHANISM; PROTEINS; Multidisciplinary Sciences

Abstract

Bacterial genomic islands are often flanked by tRNA genes, which act as sites for the integration of foreign DNA into the host chromosome. For example, Bacillus cereus ATCC14579 contains a pathogenicity island flanked by a predicted pseudo-tRNA, tRNA(Other), which does not function in translation. Deletion of tRNA(Other) led to significant changes in cell wall morphology and antibiotic resistance and was accompanied by changes in the expression of numerous genes involved in oxidative stress responses, several of which contain significant complementarities to sequences surrounding tRNA(Other). This suggested that tRNA(Other) might be expressed as part of a larger RNA, and RACE analysis subsequently confirmed the existence of several RNA species that significantly extend both the 39 and 5'-ends of tRNA(Other). tRNA(Other) expression levels were found to be responsive to changes in extracellular iron concentration, consistent with the presence of three putative ferric uptake regulator (Fur) binding sites in the 59 leader region of one of these larger RNAs. Taken together with previous data, this study now suggests that tRNA(Other) may function by providing a tRNA-like structural element within a larger regulatory RNA. These findings illustrate that while integration of genomic islands often leaves tRNA genes intact and functional, in other instances inactivation may generate tRNA-like elements that are then recruited to other functions in the cell.

Journal Title

Plos One

Volume

7

Issue/Number

7

Publication Date

1-1-2012

Document Type

Article

Language

English

First Page

10

WOS Identifier

WOS:000306548900100

ISSN

1932-6203

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