Sex differences in creatine kinase after acute heavy resistance exercise on circulating granulocyte estradiol receptors

    Authors

    M. R. Wolf; M. S. Fragala; J. S. Volek; C. R. Denegar; J. M. Anderson; B. A. Comstock; C. Dunn-Lewis; D. R. Hooper; T. K. Szivak; H. Y. Luk; C. M. Maresh; K. Hakkinen;W. J. Kraemer

    Comments

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    Abbreviated Journal Title

    Eur. J. Appl. Physiol.

    Keywords

    Resistance exercise; Tissue disruption; Estrogen; Skeletal muscle; damage; Creatine kinase; INDUCED MUSCLE DAMAGE; SKELETAL-MUSCLE; ECCENTRIC EXERCISE; GENDER; DIFFERENCES; OXIDATIVE DAMAGE; ESTROGEN; INFLAMMATION; RAT; EXPRESSION; LEAKAGE; Physiology; Sport Sciences

    Abstract

    Previous research has shown reduced tissue disruption and inflammatory responses in women as compared to men following acute strenuous exercise. While the mechanism of this action is not known, estrogen may reduce the inflammatory response through its interaction with granulocytes. The purpose of this study was to determine if estrogen receptor beta expression on granulocytes is related to sex differences in tissue disruption in response to an acute heavy resistance exercise protocol. Seven healthy, resistance-trained, eumenorrheic women (23 +/- A 3 years, 169 +/- A 9.1 cm, 66.4 +/- A 10.5 kg) and 8 healthy, resistance-trained men (25 +/- A 5 years, 178 +/- A 6.7 cm, 82.3 +/- A 9.33 kg) volunteered to participate in the study. Subjects performed an acute resistance exercise test consisting of six sets of five squats at 90% of the subject's one repetition maximum. Blood samples were obtained pre-, mid-, post-, and 1-, 6-, and 24-h postexercise. Blood samples were analyzed for 17-beta-estradiol by ELISA, creatine kinase by colorimetric enzyme immunoassay, and estradiol receptors on circulating granulocytes through flow cytometry. Men had higher CK concentrations than women at baseline/control. Men had significantly higher CK concentrations at 24-h postexercise than women. No significant changes in estradiol beta receptors were expressed on granulocytes after exercise or between sexes. While sex differences occur in CK activity in response to strenuous eccentric exercise, they may not be related to estradiol receptor beta expression on granulocytes. Thus, although there are sex differences in CK expression following acute resistance exercise, the differences may not be attributable to estrogen receptor beta expression on granulocytes.

    Journal Title

    European Journal of Applied Physiology

    Volume

    112

    Issue/Number

    9

    Publication Date

    1-1-2012

    Document Type

    Article

    Language

    English

    First Page

    3335

    Last Page

    3340

    WOS Identifier

    WOS:000307538400018

    ISSN

    1439-6319

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