Title

Sex differences in creatine kinase after acute heavy resistance exercise on circulating granulocyte estradiol receptors

Authors

Authors

M. R. Wolf; M. S. Fragala; J. S. Volek; C. R. Denegar; J. M. Anderson; B. A. Comstock; C. Dunn-Lewis; D. R. Hooper; T. K. Szivak; H. Y. Luk; C. M. Maresh; K. Hakkinen;W. J. Kraemer

Comments

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Abbreviated Journal Title

Eur. J. Appl. Physiol.

Keywords

Resistance exercise; Tissue disruption; Estrogen; Skeletal muscle; damage; Creatine kinase; INDUCED MUSCLE DAMAGE; SKELETAL-MUSCLE; ECCENTRIC EXERCISE; GENDER; DIFFERENCES; OXIDATIVE DAMAGE; ESTROGEN; INFLAMMATION; RAT; EXPRESSION; LEAKAGE; Physiology; Sport Sciences

Abstract

Previous research has shown reduced tissue disruption and inflammatory responses in women as compared to men following acute strenuous exercise. While the mechanism of this action is not known, estrogen may reduce the inflammatory response through its interaction with granulocytes. The purpose of this study was to determine if estrogen receptor beta expression on granulocytes is related to sex differences in tissue disruption in response to an acute heavy resistance exercise protocol. Seven healthy, resistance-trained, eumenorrheic women (23 +/- A 3 years, 169 +/- A 9.1 cm, 66.4 +/- A 10.5 kg) and 8 healthy, resistance-trained men (25 +/- A 5 years, 178 +/- A 6.7 cm, 82.3 +/- A 9.33 kg) volunteered to participate in the study. Subjects performed an acute resistance exercise test consisting of six sets of five squats at 90% of the subject's one repetition maximum. Blood samples were obtained pre-, mid-, post-, and 1-, 6-, and 24-h postexercise. Blood samples were analyzed for 17-beta-estradiol by ELISA, creatine kinase by colorimetric enzyme immunoassay, and estradiol receptors on circulating granulocytes through flow cytometry. Men had higher CK concentrations than women at baseline/control. Men had significantly higher CK concentrations at 24-h postexercise than women. No significant changes in estradiol beta receptors were expressed on granulocytes after exercise or between sexes. While sex differences occur in CK activity in response to strenuous eccentric exercise, they may not be related to estradiol receptor beta expression on granulocytes. Thus, although there are sex differences in CK expression following acute resistance exercise, the differences may not be attributable to estrogen receptor beta expression on granulocytes.

Journal Title

European Journal of Applied Physiology

Volume

112

Issue/Number

9

Publication Date

1-1-2012

Document Type

Article

Language

English

First Page

3335

Last Page

3340

WOS Identifier

WOS:000307538400018

ISSN

1439-6319

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