Title

Measuring anisotropic cell motility on curved substrates

Authors

Authors

K. M. Douglass; N. A. Sparrow; M. Bott; C. Fernandez-Valle;A. Dogariu

Comments

Authors: contact us about adding a copy of your work at STARS@ucf.edu

Abbreviated Journal Title

J. Biophotonics

Keywords

cell motility; mechanical stimuli; difference imaging; motility assay; MICROFILAMENT BUNDLES; EPITHELIOCYTES; MIGRATION; MECHANICS; Biochemical Research Methods; Biophysics; Optics

Abstract

Schwann cell motility was observed on laminin-coated quartz cylinders with different curvatures over an 18 hour period. A new analysis based on difference images helped to determine the minimal radius of curvature, 46 m, which restricted motility along the cylinder axis. The migration speed, measured by calculating differences between successive images in the time series, ranged between 0.3 to 0.8 m per minute and is similar to previously reported rates for Schwann cells. Difference images provide a rapid and simple method for the analysis of cell motility on large populations of cells. ((c) 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

Journal Title

Journal of Biophotonics

Volume

6

Issue/Number

5

Publication Date

1-1-2013

Document Type

Article

Language

English

First Page

387

Last Page

392

WOS Identifier

WOS:000318363000004

ISSN

1864-063X

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