Measuring anisotropic cell motility on curved substrates

    Authors

    K. M. Douglass; N. A. Sparrow; M. Bott; C. Fernandez-Valle;A. Dogariu

    Comments

    Authors: contact us about adding a copy of your work at STARS@ucf.edu

    Abbreviated Journal Title

    J. Biophotonics

    Keywords

    cell motility; mechanical stimuli; difference imaging; motility assay; MICROFILAMENT BUNDLES; EPITHELIOCYTES; MIGRATION; MECHANICS; Biochemical Research Methods; Biophysics; Optics

    Abstract

    Schwann cell motility was observed on laminin-coated quartz cylinders with different curvatures over an 18 hour period. A new analysis based on difference images helped to determine the minimal radius of curvature, 46 m, which restricted motility along the cylinder axis. The migration speed, measured by calculating differences between successive images in the time series, ranged between 0.3 to 0.8 m per minute and is similar to previously reported rates for Schwann cells. Difference images provide a rapid and simple method for the analysis of cell motility on large populations of cells. ((c) 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

    Journal Title

    Journal of Biophotonics

    Volume

    6

    Issue/Number

    5

    Publication Date

    1-1-2013

    Document Type

    Article

    Language

    English

    First Page

    387

    Last Page

    392

    WOS Identifier

    WOS:000318363000004

    ISSN

    1864-063X

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