Phosphate ester hydrolysis of biologically relevant molecules by cerium oxide nanoparticles

    Authors

    M. H. Kuchma; C. B. Komanski; J. Colon; A. Teblum; A. E. Masunov; B. Alvarado; S. Babu; S. Seal; J. Summy;C. H. Baker

    Comments

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    Abbreviated Journal Title

    Nanomed.-Nanotechnol. Biol. Med.

    Keywords

    Cerium oxide nanoparticle; Phosphate ester hydrolysis; Nanoceria; Phosphatase mimetic; Dephosphorylation; DNA HYDROLYSIS; TRANSITION-STATES; BOND-CLEAVAGE; PROTECTION; COMPLEXES; CATALYSTS; DIESTERS; IONS; RNA; Nanoscience & Nanotechnology; Medicine, Research & Experimental

    Abstract

    In an effort to characterize the interaction of cerium oxide nanoparticles (CNPs) in biological systems, we explored the reactivity of CNPs with the phosphate ester bonds of p-nitrophenylphosphate (pNPP), ATP, o-phospho-L-tyrosine, and DNA. The activity of the bond cleavage for pNPP at pH 7 is calculated to be 0.860 +/- 0.010 nmol p-nitrophenol/min/mu g CNPs. Interestingly, when CNPs bind to plasmid DNA, no cleavage products are detected. While cerium(IV) complexes generally exhibit the ability to break phosphorus-oxygen bonds, the reactions we report appear to be dependent on the availability of cerium(III) sites, not cerium(IV) sites. We investigated the dephosphorylation mechanism from the first principles and find the reaction proceeds through inversion of the phosphate group similar to an S(N)2 mechanism. The ability of CNPs to interact with phosphate ester bonds of biologically relevant molecules has important implications for their use as potential therapeutics. From the Clinical Editor: The ability of cerium oxide nanoparticles to interact with phosphate ester bonds of biologically relevant molecules has important implications for their use as potential therapeutics. This team of investigators explored the reactivity of these nanoparticles with the phosphate ester bonds of p-nitrophenylphosphate, ATP, o-phospho-L-tyrosine, and DNA. (C) 2010 Elsevier Inc. All rights reserved.

    Journal Title

    Nanomedicine-Nanotechnology Biology and Medicine

    Volume

    6

    Issue/Number

    6

    Publication Date

    1-1-2010

    Document Type

    Article

    Language

    English

    First Page

    738

    Last Page

    744

    WOS Identifier

    WOS:000284943200004

    ISSN

    1549-9634

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