Title

Phosphate ester hydrolysis of biologically relevant molecules by cerium oxide nanoparticles

Authors

Authors

M. H. Kuchma; C. B. Komanski; J. Colon; A. Teblum; A. E. Masunov; B. Alvarado; S. Babu; S. Seal; J. Summy;C. H. Baker

Comments

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Abbreviated Journal Title

Nanomed.-Nanotechnol. Biol. Med.

Keywords

Cerium oxide nanoparticle; Phosphate ester hydrolysis; Nanoceria; Phosphatase mimetic; Dephosphorylation; DNA HYDROLYSIS; TRANSITION-STATES; BOND-CLEAVAGE; PROTECTION; COMPLEXES; CATALYSTS; DIESTERS; IONS; RNA; Nanoscience & Nanotechnology; Medicine, Research & Experimental

Abstract

In an effort to characterize the interaction of cerium oxide nanoparticles (CNPs) in biological systems, we explored the reactivity of CNPs with the phosphate ester bonds of p-nitrophenylphosphate (pNPP), ATP, o-phospho-L-tyrosine, and DNA. The activity of the bond cleavage for pNPP at pH 7 is calculated to be 0.860 +/- 0.010 nmol p-nitrophenol/min/mu g CNPs. Interestingly, when CNPs bind to plasmid DNA, no cleavage products are detected. While cerium(IV) complexes generally exhibit the ability to break phosphorus-oxygen bonds, the reactions we report appear to be dependent on the availability of cerium(III) sites, not cerium(IV) sites. We investigated the dephosphorylation mechanism from the first principles and find the reaction proceeds through inversion of the phosphate group similar to an S(N)2 mechanism. The ability of CNPs to interact with phosphate ester bonds of biologically relevant molecules has important implications for their use as potential therapeutics. From the Clinical Editor: The ability of cerium oxide nanoparticles to interact with phosphate ester bonds of biologically relevant molecules has important implications for their use as potential therapeutics. This team of investigators explored the reactivity of these nanoparticles with the phosphate ester bonds of p-nitrophenylphosphate, ATP, o-phospho-L-tyrosine, and DNA. (C) 2010 Elsevier Inc. All rights reserved.

Journal Title

Nanomedicine-Nanotechnology Biology and Medicine

Volume

6

Issue/Number

6

Publication Date

1-1-2010

Document Type

Article

Language

English

First Page

738

Last Page

744

WOS Identifier

WOS:000284943200004

ISSN

1549-9634

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