Title

Identifying Parentage Using Molecular Markers: Improving Accuracy of Studbook Records for a Captive Flock of Marabou Storks (Leptoptilus crumeniferus)

Authors

Authors

G. M. Ferrie; O. R. Cohen; P. Schutz; K. A. Leighty; C. Plasse; T. L. Bettinger;E. A. Hoffman

Comments

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Abbreviated Journal Title

Zoo Biol.

Keywords

captive breeding; extra-pair paternity; microsatellite; parentage; pedigree; studbook; EXTRA-PAIR COPULATIONS; BREEDING BIOLOGY; COMPUTER-PROGRAM; BIRDS; PATERNITY; EVOLUTION; DENSITY; ERRORS; DNA; Veterinary Sciences; Zoology

Abstract

Extra-pair copulations (EPCs) leading to extra-pair fertilization (EPF) are common in avian mating systems, despite the prevalence of observed social monogamy in many species. Colonially breeding birds are interesting species to investigate the prevalence of EPCs and EPF because they show nesting habits including close proximity of nest sites and sexual partners, which are proposed to promote alternative reproductive tactics. Endemic to Africa, the colonial marabou stork (Leptoptilos crumeniferus) is one of the most commonly held avian species in North American zoos. The aims of this study were to use genetic information to verify parentage in a population of marabou stork housed at Disney's Animal Kingdom (R) based on five microsatellite loci and to investigate reproductive behavior. We compared genetic analyses of parents and offspring to studbook data collected through behavioral observations of parental behavior at the nest. Using genetic analyses to reconstruct the pedigree of the marabou stork flock using the program COLONY led to improvement of studbook records by determining parentage of an individual that had previously unknown parentage, and identified one individual that had a sire that differed genetically from studbook records. An important contribution of our analyses was the identification and verification of the most likely parents for offspring hatched in this colony and improving incorrect or undocumented parentage in the studbook. Additionally, the colonial nature of this species makes it difficult to observe and understand reproductive behavior. Gaining better understanding of the mating system of a species is essential for successful breeding and captive management. Zoo Biol. 32:556-564, 2013. (c) 2013 Wiley Periodicals, Inc.

Journal Title

Zoo Biology

Volume

32

Issue/Number

5

Publication Date

1-1-2013

Document Type

Article

Language

English

First Page

556

Last Page

564

WOS Identifier

WOS:000325978100011

ISSN

0733-3188

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