Analysis of polycyclic aromatic hydrocarbon metabolites in cow's milk by liquid-liquid extraction and synchronous room-temperature fluorescence spectroscopy

    Authors

    G. Knobel; K. Calimag-Williams;A. D. Campiglia

    Comments

    Authors: contact us about adding a copy of your work at STARS@ucf.edu

    Abbreviated Journal Title

    Anal. Methods

    Keywords

    FISH BILE; EMISSION SPECTRA; EXPOSURE; EXCITATION; URINE; 1-HYDROXYPYRENE; LUMINESCENCE; MATRICES; SAMPLES; SOIL; Chemistry, Analytical; Food Science & Technology; Spectroscopy

    Abstract

    We present the first application of synchronous room-temperature fluorescence spectroscopy to the analysis of monohydroxy-polycyclic aromatic hydrocarbons in milk samples. Metabolites are extracted from milk samples by a two-step liquid-liquid extraction procedure. The small sample (400 mu l) and extracting solvent (1.4 ml) volumes facilitate the simultaneous extraction of numerous samples via an environmentally friendly procedure, which is well-suited for routine monitoring of numerous samples. Quantitative analysis is carried out in the sample extract without the need for previous chromatographic separation. Excellent recoveries and limits of detection at the parts-per-billion level were obtained for 2-hydroxyfluorene, 6-hydroxychrysene, 1-hydroxypyrene and 3-hydroxybenzo[a]pyrene. The non-destructive nature of the new method provides ample opportunity for metabolite confirmation via chromatographic techniques.

    Journal Title

    Analytical Methods

    Volume

    5

    Issue/Number

    6

    Publication Date

    1-1-2013

    Document Type

    Article

    Language

    English

    First Page

    1577

    Last Page

    1582

    WOS Identifier

    WOS:000315508900029

    ISSN

    1759-9660

    Share

    COinS