Determination of polycyclic aromatic hydrocarbon metabolites in milk by a quick, easy, cheap, effective, rugged and safe extraction and capillary electrophoresis

    Authors

    G. Knobel;A. D. Campiglia

    Comments

    Authors: contact us about adding a copy of your work at STARS@ucf.edu

    Abbreviated Journal Title

    J. Sep. Sci.

    Keywords

    Capillary electrophoresis; Metabolites; Milk; Polycyclic Aromatic; Hydrocarbons; SOLID-PHASE EXTRACTION; MICELLAR ELECTROKINETIC CHROMATOGRAPHY; TEMPERATURE FLUORESCENCE SPECTROSCOPY; PHOTOIONIZATION-MASS-SPECTROMETRY; POLYAROMATIC HYDROCARBONS; SAMPLE; STACKING; ZONE-ELECTROPHORESIS; GOLD NANOPARTICLES; QUECHERS METHOD; MATRICES; Chemistry, Analytical

    Abstract

    We describe the first application of a quick, easy, cheap, effective, rugged and safe extraction technique to the CZE analysis of monohydroxylated metabolites of polycyclic aromatic hydrocarbons in milk. Complete resolution of 2-hydroxyfluorenene, 1-hydroxynaphthalene, 2-hydroxynaphthalene, 3-hydroxyphenanthrene, and 9-hydroxyphenanthrene was accomplished in 4 min of electrophoretic run. Limits of detection at the parts-per-billion were obtained with a single solvent (acetonitrile) for metabolite extraction and sample stacking. The small sample volume (1.2 mL) and the conservative usage of chemicals provided a simple and rapid procedure for the simultaneous extraction of numerous samples. Adding 4 min of electrophoretic run per sample, it should be possible to screen ten samples in approximately 1 h of analysis time. The nanoliter extract volume required for sample injection allows for further chromatographic usage and confirmation of positive samples. The unique electrophoretic pattern of the studied metabolites demonstrates the potential for the unambiguous determination of positional isomers with very similar chromatographic behaviors and undistinguishable mass fragmentation patterns.

    Journal Title

    Journal of Separation Science

    Volume

    36

    Issue/Number

    14

    Publication Date

    1-1-2013

    Document Type

    Article

    Language

    English

    First Page

    2291

    Last Page

    2298

    WOS Identifier

    WOS:000321760600010

    ISSN

    1615-9306

    Share

    COinS