Responses of proenkephalin Peptide F to aerobic exercise stress in the plasma and white blood cell biocompartments

    Authors

    W. J. Kraemer; M. S. Fragala; Wrhb van Henegouwen; S. E. Gordon; J. A. Bush; J. S. Volek; N. T. Triplett; C. Dunn-Lewis; B. A. Comstock; T. K. Szivak; S. D. Flanagan; D. R. Hooper; H. Y. Luk;A. M. Mastro

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    Abbreviated Journal Title

    Peptides

    Keywords

    Enkephalin-containing peptides; Opioid responses to exercise; Buffy; coat; Exercise; Immune function; GRADED-EXERCISE; MEN; Biochemistry & Molecular Biology; Pharmacology & Pharmacy

    Abstract

    Proenkephalin Peptide F [107-140] is an enkephalin-containing peptide found predominantly within the adrenal medulla, co-packaged with epinephrine within the chromaffin granules. In vivo studies indicate that Peptide F has classic opioid analgesia effects: in vitro studies suggest potential immune cell interactions. In this investigation we examined patterns of Peptide F concentrations in different bio-compartmyents of the blood at rest and following sub-maximal cycle exercise to determine if Peptide F interacts with the white blood cell (WBC) bio-compartment during aerobic exercise. Eight physically active men (n = 8) performed sub-maximal (80-85% (V) over dotO(2) (peak)) cycle ergometer exercise for 30 min. Plasma Peptide F and WBC Peptide F immunoreactivity were examined pre-exercise, mid-exercise and immediately post-, 5-min post-, 15-min post-, 30-min post- and 60-min post-exercise and at similar time-points during a control condition (30 min rest). Peptide F concentrations significantly (p < 0.05) increased at 5 and 60 min post-exercise, compared to pre-exercise concentrations. No significant increases in Peptide F concentrations in the WBC fraction were observed during or after exercise. However, a significant decrease was observed at 30 min post-exercise. An ultradian pattern of Peptide F distribution was apparent during rest. Furthermore, concentrations of T cells, B cells, NK cells, and total WBCs demonstrated significant changes in response to aerobic exercise. Data indicated that Peptide F was bound in significant molar concentrations in the WBC fraction and that this biocompartment may be one of the tissue targets for binding interactions. These data indicate that Peptide F is involved with immune cell modulation in the white blood circulatory biocompartment of blood. (C) 2013 Published by Elsevier Inc.

    Journal Title

    Peptides

    Volume

    42

    Publication Date

    1-1-2013

    Document Type

    Article

    Language

    English

    First Page

    118

    Last Page

    124

    WOS Identifier

    WOS:000320492800018

    ISSN

    0196-9781

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