Title

Four-Way Junction Formation Promoting Ultrasensitive Electrochemical Detection of MicroRNA

Authors

Authors

M. Labib; S. M. Ghobadloo; N. Khan; D. M. Kolpashchikov;M. V. Berezovski

Comments

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Abbreviated Journal Title

Biochim. Biophys. Acta-Gen. Subj.

Keywords

CIRCULATING MICRORNAS; PLASMA; PROBE; BIOSENSORS; SEQUENCES; IMPEDANCE; SENSORS; MARKERS; Chemistry, Analytical

Abstract

MicroRNAs (miRNAs) represent a class of biomarkers that are frequently deregulated in cancer cells and have shown a great promise for cancer classification and prognosis. Here, we endeavored to develop a DNA four-way junction based electrochemical sensor (4J-SENS) for ultrasensitive miRNA analysis. The developed sensor can be operated within the dynamic range from 10 aM to 1 fM and detect as low as 2 aM of miR-122 (similar to 36 molecules per sample), without PCR amplification. Furthermore, the 4J-SENS was employed to profile endogenouse hsa-miR-122 in healthy human and chronic lymphocyitc leukemia (CLL) patient serum, and the results were validated by qPCR analysis.

Journal Title

Analytical Chemistry

Volume

Anal. Chem.

Issue/Number

20

Publication Date

1-1-2013

Document Type

Article

Language

English

First Page

9422

Last Page

9427

WOS Identifier

85

ISSN

0003-2700

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