Four-Way Junction Formation Promoting Ultrasensitive Electrochemical Detection of MicroRNA

    Authors

    M. Labib; S. M. Ghobadloo; N. Khan; D. M. Kolpashchikov;M. V. Berezovski

    Comments

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    Abbreviated Journal Title

    Biochim. Biophys. Acta-Gen. Subj.

    Keywords

    CIRCULATING MICRORNAS; PLASMA; PROBE; BIOSENSORS; SEQUENCES; IMPEDANCE; SENSORS; MARKERS; Chemistry, Analytical

    Abstract

    MicroRNAs (miRNAs) represent a class of biomarkers that are frequently deregulated in cancer cells and have shown a great promise for cancer classification and prognosis. Here, we endeavored to develop a DNA four-way junction based electrochemical sensor (4J-SENS) for ultrasensitive miRNA analysis. The developed sensor can be operated within the dynamic range from 10 aM to 1 fM and detect as low as 2 aM of miR-122 (similar to 36 molecules per sample), without PCR amplification. Furthermore, the 4J-SENS was employed to profile endogenouse hsa-miR-122 in healthy human and chronic lymphocyitc leukemia (CLL) patient serum, and the results were validated by qPCR analysis.

    Journal Title

    Analytical Chemistry

    Volume

    Anal. Chem.

    Issue/Number

    20

    Publication Date

    1-1-2013

    Document Type

    Article

    Language

    English

    First Page

    9422

    Last Page

    9427

    WOS Identifier

    85

    ISSN

    0003-2700

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