Title

Caveolae-Mediated Endocytosis of Conjugated Polymer Nanoparticles

Authors

Authors

J. H. Lee; M. Twomey; C. Machado; G. Gomez; M. Doshi; A. J. Gesquiere;J. H. Moon

Comments

Authors: contact us about adding a copy of your work at STARS@ucf.edu

Abbreviated Journal Title

Med. Sci. Sports Exerc.

Keywords

conjugated polyelectrolytes; conjugated polymers; conjugated polymer; nanoparticles; endocytosis mechanism; small interfering RNA delivery; INTRACELLULAR TRAFFICKING; SIRNA DELIVERY; GENE DELIVERY; DOTS; PATHWAYS; CARRIERS; CELLS; Biochemistry & Molecular Biology; Materials Science, Biomaterials; Polymer Science

Abstract

Understanding the cellular entry pathways of synthetic biomaterials is highly important to improve overall labeling and delivery efficiency. Herein, cellular entry mechanisms of conjugated polymer nanoparticles (CPNs) are presented. CPNs are intrinsic fluorescent materials used for various biological applications. While CPNs cause no toxicity, decreased CPN uptake is observed from cancer cells pretreated with genistein, which is an inhibitor of caveolae-mediated endocytosis (CvME). CvME is further confirmed by high co-localization with caveolin-1 proteins found in the caveolae and caveosomes. Excellent photophysical properties, non-toxicity, and non-destructive delivery pathways support that CPNs are promising multifunctional carriers minimizing degradation of contents during delivery.

Journal Title

Macromolecular Bioscience

Volume

Macromol. Biosci.

Issue/Number

7

Publication Date

1-1-2013

Document Type

Article

Language

English

First Page

913

Last Page

920

WOS Identifier

13

ISSN

1616-5187

Share

COinS