Caveolae-Mediated Endocytosis of Conjugated Polymer Nanoparticles

    Authors

    J. H. Lee; M. Twomey; C. Machado; G. Gomez; M. Doshi; A. J. Gesquiere;J. H. Moon

    Comments

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    Abbreviated Journal Title

    Med. Sci. Sports Exerc.

    Keywords

    conjugated polyelectrolytes; conjugated polymers; conjugated polymer; nanoparticles; endocytosis mechanism; small interfering RNA delivery; INTRACELLULAR TRAFFICKING; SIRNA DELIVERY; GENE DELIVERY; DOTS; PATHWAYS; CARRIERS; CELLS; Biochemistry & Molecular Biology; Materials Science, Biomaterials; Polymer Science

    Abstract

    Understanding the cellular entry pathways of synthetic biomaterials is highly important to improve overall labeling and delivery efficiency. Herein, cellular entry mechanisms of conjugated polymer nanoparticles (CPNs) are presented. CPNs are intrinsic fluorescent materials used for various biological applications. While CPNs cause no toxicity, decreased CPN uptake is observed from cancer cells pretreated with genistein, which is an inhibitor of caveolae-mediated endocytosis (CvME). CvME is further confirmed by high co-localization with caveolin-1 proteins found in the caveolae and caveosomes. Excellent photophysical properties, non-toxicity, and non-destructive delivery pathways support that CPNs are promising multifunctional carriers minimizing degradation of contents during delivery.

    Journal Title

    Macromolecular Bioscience

    Volume

    Macromol. Biosci.

    Issue/Number

    7

    Publication Date

    1-1-2013

    Document Type

    Article

    Language

    English

    First Page

    913

    Last Page

    920

    WOS Identifier

    13

    ISSN

    1616-5187

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