Targeted nanoparticles for enhanced X-ray radiation killing of multidrug-resistant bacteria

    Authors

    Y. Luo; M. Hossain; C. M. Wang; Y. Qiao; J. C. An; L. Y. Ma;M. Su

    Comments

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    Abbreviated Journal Title

    Nanoscale

    Keywords

    BISMUTH SULFIDE NANOPARTICLES; GOLD NANOPARTICLES; PATHOGENIC BACTERIA; PHOTOTHERMAL AGENTS; DOSE ENHANCEMENT; BREAST-CANCER; IRRADIATION; DNA; CYTOTOXICITY; CELLS; Chemistry, Multidisciplinary; Nanoscience & Nanotechnology; Materials; Science, Multidisciplinary; Physics, Applied

    Abstract

    This paper describes a nanoparticle enhanced X-ray irradiation based strategy that can be used to kill multidrug resistant (MDR) bacteria. In the proof-of-concept experiment using MDR Pseudomonas aeruginosa (P. aeruginosa) as an example, polyclonal antibody modified bismuth nanoparticles are introduced into bacterial culture to specifically target P. aeruginosa. After washing off uncombined bismuth nanoparticles, the bacteria are irradiated with X-rays, using a setup that mimics a deeply buried wound in humans. Results show that up to 90% of MDR P. aeruginosa are killed in the presence of 200 mu g ml(-1) bismuth nanoparticles, whereas only similar to 6% are killed in the absence of bismuth nanoparticles when exposed to 40 kVp X-rays for 10 min. The 200 mu g ml(-1) bismuth nanoparticles enhance localized X-ray dose by 35 times higher than the control with no nanoparticles. In addition, no significant harmful effects on human cells (HeLa and MG-63 cells) have been observed with 200 mu g ml(-1) bismuth nanoparticles and 10 min 40 kVp X-ray irradiation exposures, rendering the potential for future clinical use. Since X-rays can easily penetrate human tissues, this bactericidal strategy has the potential to be used in effectively killing deeply buried MDR bacteria in vivo.

    Journal Title

    Nanoscale

    Volume

    5

    Issue/Number

    2

    Publication Date

    1-1-2013

    Document Type

    Article

    Language

    English

    First Page

    687

    Last Page

    694

    WOS Identifier

    WOS:000313426200033

    ISSN

    2040-3364

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