Predicting Efficacy of Cancer Cell Killing under Hypoxic Conditions with Single Cell DNA Damage Assay

    Authors

    Y. Qiao;L. Y. Ma

    Comments

    Authors: contact us about adding a copy of your work at [email protected]

    Abbreviated Journal Title

    Anal. Chem.

    Keywords

    IN-VITRO; BREAST-CANCER; TUMOR HYPOXIA; ANTITUMOR-ACTIVITY; DRUG-SENSITIVITY; RESISTANCE; CHEMOSENSITIVITY; CHEMOTHERAPY; OXYGEN; HETEROGENEITY; Chemistry, Analytical

    Abstract

    The activity of anticancer drugs determined under normal conditions cannot accurately reflect true drug efficacy in a patient, as a tumor is often under low oxygen (hypoxia) conditions. In addition, patient responses to the same therapy can be drastically different due to tumor heterogeneity. This paper describes the use of single cell halo assay for detection and quantification of DNA damage induced by anticancer drugs or radiation under hypoxic conditions. By combining classical halo assay and state-of-the-art microfabrication techniques, this single cell approach allows drug and radiation responses of cancer cells to be determined without population interference. The results from single cell assay indicate a diminished level of DNA damage at hypoxic conditions compared with those at normal conditions at the same drug concentrations or radiation dose, suggesting in vitro preclinical studies of drug and radiation activity can be performed under conditions that mimic physiological conditions of tumors and without population interference.

    Journal Title

    Analytical Chemistry

    Volume

    85

    Issue/Number

    14

    Publication Date

    1-1-2013

    Document Type

    Article

    Language

    English

    First Page

    6953

    Last Page

    6957

    WOS Identifier

    WOS:000322059600058

    ISSN

    0003-2700

    Share

    COinS
     

    Accessibility Statement

    This item was created or digitized prior to April 24, 2027, or is a reproduction of legacy media created before that date. It is preserved in its original, unmodified state specifically for research, reference, or historical recordkeeping. In accordance with the ADA Title II Final Rule, the University Libraries provides accessible versions of archival materials upon request. To request an accommodation for this item, please submit an accessibility request form.